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Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei
Zhang, Haofang1,2,3; Li, Shihao1,2,4; Wang, Fuxuan1; Xiang, Jianhai1,2; Li, Fuhua1,2,4,5
2020-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume109Pages:9
AbstractLeucine-rich repeat (LRR) is a vital structure in some pattern recognition receptors such as TLRs, NLRs and newly reported LRR-containing proteins. Apart from some limited reported LRR-containing proteins, most of LRR proteins, especially immune-related proteins, remain uncharacterized functionally. In the present study, a transmembrane protein containing several LRR motifs, designated as LvLRRm, was identified from the shrimp Litopenaeus vannamei. LvLRRm contained a long signal peptide, one LRRNT region, 12 LRR motifs, one LRRCT region and a transmembrane region. The transcripts of LvLRRm were widely distributed in all tested tissues of shrimp and they were responsive to Vibrio parahaemolyticus infection in several immune-related tissues including Oka, intestine, gill and hemocytes. Knockdown of LvLRRm by dsRNA interference led to a decreased survival rate of shrimp infected by Vibrio parahaemolyticus and an increased in vivo Vibrio propagation. Meanwhile, knockdown of LvLRRm also down-regulated the expression levels of genes involved in antibacterial immune signaling pathways, including the transcription factors LvDorsal and LvRelish, and several antimicrobial peptides. These data suggested that LvLRRm played important roles in shrimp against Vibrio infection, which was probably functioning through activation of antibacterial immune signaling pathways. The present study provided new evidence to elucidate the immune function of LRR-containing proteins in invertebrates.
KeywordLeucine-rich repeat RNA interference Vibrio parahaemolyticus Innate immunity Signaling pathway
DOI10.1016/j.dci.2020.103713
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48[CARS-48]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000532168100017
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/167374
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Hubei, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Haofang,Li, Shihao,Wang, Fuxuan,et al. Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,109:9.
APA Zhang, Haofang,Li, Shihao,Wang, Fuxuan,Xiang, Jianhai,&Li, Fuhua.(2020).Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,109,9.
MLA Zhang, Haofang,et al."Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 109(2020):9.
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