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Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells
Li, Xiaosong1,2,3; Wu, Ning1,2; Chen, Yongqiang4; Tan, Jiaojiao1,2,3; Wang, Jing1,2; Geng, Lihua1,2; Qin, Yimin5; Zhang, Quanbin1,2,3
2020-05-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume151Pages:545-553
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractIn order to investigate the anti-fibrotic effect of different molecular weight (Mw) fucoidans on TGF-beta 1-induced mouse renal tubular epithelial cell (MTEC) mode. Oxidative degradation method was used to obtain fucoidans with different molecular weights and the reaction time, reaction temperature and the concentration of oxidants were investigated. Cell viability was detected by CCK-8, and EMT markers expression was detected by Westernbolt and Cell immunofluorescence assay. As a result, after chemical analysis of three independent batches of prepared samples, one batch of fucoidan sample (LHX 1-9) which chemical contents are similar but Mw ranging from 3.3 KDa to 49.3 KDa were selected to do further research. We found LHX1 (Mw - 3.3 KDa) and LHX 3-9 (Mw = 6.6 KDa, 8.3 KDa, 11.3 KDa, 14.9 KDa, 25.2 KDa, 35.4 KDa, 49.3 KDa) could resist the TGF-beta 1-induced depithelial-mesenchymal transition (EMT) by decreased expression of Fn and CTGF and maintained epithelial cell morphology in MTEC. However, the relationship between the Mw of fucoidans and their anti-EMT effect is not simply linear. Among the samples, LHX 1, 5 and 8 showed significant anti-EMT effects than others by deregulated Fn and CTGF expression on MTEC cells. (C) 2020 Elsevier B.V. All rights reserved.
KeywordDegradation Fucoidan Epithelial-mesenchymal transition Kidney fibrosis
DOI10.1016/j.ijbiomac.2020.02.068
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0901104] ; Major Scientific & Engineering Projects of Innovation in Shandong Province[2019JZZY010818] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2016190] ; K. C. Wong Education Foundation, CAS
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000528267400056
PublisherELSEVIER
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167329
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Eighth Peoples Hosp, Anorectal Surg, Qingdao 266100, Peoples R China
5.State Key Lab Bioact Seaweed Subst, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xiaosong,Wu, Ning,Chen, Yongqiang,et al. Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,151:545-553.
APA Li, Xiaosong.,Wu, Ning.,Chen, Yongqiang.,Tan, Jiaojiao.,Wang, Jing.,...&Zhang, Quanbin.(2020).Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,151,545-553.
MLA Li, Xiaosong,et al."Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 151(2020):545-553.
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