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Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells
Li, Xiaosong1,2,3; Wu, Ning1,2; Chen, Yongqiang4; Tan, Jiaojiao1,2,3; Wang, Jing1,2; Geng, Lihua1,2; Qin, Yimin5; Zhang, Quanbin1,2,3
2020-05-15
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号151页码:545-553
通讯作者Zhang, Quanbin(qbzhang@qdio.ac.cn)
摘要In order to investigate the anti-fibrotic effect of different molecular weight (Mw) fucoidans on TGF-beta 1-induced mouse renal tubular epithelial cell (MTEC) mode. Oxidative degradation method was used to obtain fucoidans with different molecular weights and the reaction time, reaction temperature and the concentration of oxidants were investigated. Cell viability was detected by CCK-8, and EMT markers expression was detected by Westernbolt and Cell immunofluorescence assay. As a result, after chemical analysis of three independent batches of prepared samples, one batch of fucoidan sample (LHX 1-9) which chemical contents are similar but Mw ranging from 3.3 KDa to 49.3 KDa were selected to do further research. We found LHX1 (Mw - 3.3 KDa) and LHX 3-9 (Mw = 6.6 KDa, 8.3 KDa, 11.3 KDa, 14.9 KDa, 25.2 KDa, 35.4 KDa, 49.3 KDa) could resist the TGF-beta 1-induced depithelial-mesenchymal transition (EMT) by decreased expression of Fn and CTGF and maintained epithelial cell morphology in MTEC. However, the relationship between the Mw of fucoidans and their anti-EMT effect is not simply linear. Among the samples, LHX 1, 5 and 8 showed significant anti-EMT effects than others by deregulated Fn and CTGF expression on MTEC cells. (C) 2020 Elsevier B.V. All rights reserved.
关键词Degradation Fucoidan Epithelial-mesenchymal transition Kidney fibrosis
DOI10.1016/j.ijbiomac.2020.02.068
收录类别SCI
语种英语
资助项目National Key Research and Development Program of China[2018YFD0901104] ; Major Scientific & Engineering Projects of Innovation in Shandong Province[2019JZZY010818] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2016190] ; K. C. Wong Education Foundation, CAS
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号WOS:000528267400056
出版者ELSEVIER
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167329
专题实验海洋生物学重点实验室
通讯作者Zhang, Quanbin
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Eighth Peoples Hosp, Anorectal Surg, Qingdao 266100, Peoples R China
5.State Key Lab Bioact Seaweed Subst, Qingdao, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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Li, Xiaosong,Wu, Ning,Chen, Yongqiang,et al. Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,151:545-553.
APA Li, Xiaosong.,Wu, Ning.,Chen, Yongqiang.,Tan, Jiaojiao.,Wang, Jing.,...&Zhang, Quanbin.(2020).Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,151,545-553.
MLA Li, Xiaosong,et al."Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 151(2020):545-553.
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