Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot

doi:10.3964/j.issn.1000-0593(2020)03-0755-05

IOCAS-IR > 海洋地质与环境重点实验室

	Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot
	Liang Zheng-wei1,2,4,5 ; Du Zeng-feng 1,2,5; Li Chao-lun1,2,4,5 ; Wang Min-xiao 1,2,4,5; Wang Bing 1,2,5; Zhang Xin 1,2,3,4,5; Yan Jun 1,2
	2020-03-01
发表期刊	SPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN	1000-0593
卷号	40 期号:3 页码:755-759
通讯作者	Zhang Xin(xzhang@qdio.ac.cn)
摘要	Mussels are widely distributed in various marine environments, from shallow seas to cold seeps and hydrothermal vents in the deep oceans, because of its excellent adaptabilityin different physicochemical conditions. Mussels anchor themselves on the tough substrate exposed on the seabed, such authigenic carbonate crusts or rock outcrops with adhesive protein secreted by mussel foot. This protein is a natural renewable biological adhesive available in various marine environments and mussel byssus presents such as high adhesive, high toughness and resistance to water. The adhesive protein has great potential and application prospect in biotechnology and medicine field, and it has become a research hotspot nowadays. Raman spectroscopy is a kind of non-destructive and non-invasive detection technique which can provide molecular biochemical information of organisms. In this paper, SEM and confocal Raman micro-spectroscopy are used to investigatethe apparent difference of mussel foot byssus, the secreted protein composition and distribution characteristics of mussel foot gland. Based on the apparent morphological differences of deep-sea mussels and sallow-sea mussels by SEM, two kind of mussels foot tissues are detected with confocal Raman micro-spectroscopy and acquire Raman spectra and 2D Raman color-coded image of three glands. The components and relative distribution of three glands of two mussels are compared and byssus differences caused by the glands distribution are analyzed. Meanwhile, considering the living environments of the two kind of mussels, we think that the mussel byssus appearance difference and foot glands distribution is a kind of adaptation mechanism to the environment. The Raman spectra suggest the components of two mussels foot glands: the higher intensity at 1 242 and 1 269 cm(-1) to the other two peaks (1 318 and 1 337 cm (-1)) of the amide III signals indicates that the core gland is a higher degree of conformational order protein. The proteins that comprise theplaque and cuticle enrich amino acid tyrosine (Tyr) at 643, 830, 850 and 1 615 cm(-1) and post-translationally converted to 3 ,4-dihydroxyphenylalanine (DOPA) at 785 cm(-1); shallow-sea mussels present high intensity collagen protein at 1 043 cm(-1). Raman imaging shows the glands distribution feature: deep-sea mussels foot glands distribution concentration and shallow-sea mussels foot glands distribution dispersion, which suggests that the glands distribution may be a kind of mechanism for the mussels to adapt different environments. The results indicate that confocal Raman micro-spectroscopy can be used in the analysis of the mussel foot glands distribution characteristics in different environments, and has great prospect to be applied in the micro-analysis of the biological samples.
关键词	Confocal Raman spectroscopy Mussel foot gland Adhesive protein
DOI	10.3964/j.issn.1000-0593(2020)03-0755-05
收录类别	SCI
语种	英语
WOS研究方向	Spectroscopy
WOS类目	Spectroscopy
WOS记录号	WOS:000528837900015
出版者	OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167314
专题	海洋地质与环境重点实验室
通讯作者	Zhang Xin
作者单位	1.Chinese Acad Sci, CAS Key Lab Marine Geol & Environm, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao 266061, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liang Zheng-wei,Du Zeng-feng,Li Chao-lun,et al. Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2020,40(3):755-759.
APA	Liang Zheng-wei.,Du Zeng-feng.,Li Chao-lun.,Wang Min-xiao.,Wang Bing.,...&Yan Jun.(2020).Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot.SPECTROSCOPY AND SPECTRAL ANALYSIS,40(3),755-759.
MLA	Liang Zheng-wei,et al."Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot".SPECTROSCOPY AND SPECTRAL ANALYSIS 40.3(2020):755-759.

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