IOCAS-IR  > 海洋地质与环境重点实验室
Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot
Liang Zheng-wei1,2,4,5; Du Zeng-feng1,2,5; Li Chao-lun1,2,4,5; Wang Min-xiao1,2,4,5; Wang Bing1,2,5; Zhang Xin1,2,3,4,5; Yan Jun1,2
2020-03-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume40Issue:3Pages:755-759
Corresponding AuthorZhang Xin(xzhang@qdio.ac.cn)
AbstractMussels are widely distributed in various marine environments, from shallow seas to cold seeps and hydrothermal vents in the deep oceans, because of its excellent adaptabilityin different physicochemical conditions. Mussels anchor themselves on the tough substrate exposed on the seabed, such authigenic carbonate crusts or rock outcrops with adhesive protein secreted by mussel foot. This protein is a natural renewable biological adhesive available in various marine environments and mussel byssus presents such as high adhesive, high toughness and resistance to water. The adhesive protein has great potential and application prospect in biotechnology and medicine field, and it has become a research hotspot nowadays. Raman spectroscopy is a kind of non-destructive and non-invasive detection technique which can provide molecular biochemical information of organisms. In this paper, SEM and confocal Raman micro-spectroscopy are used to investigatethe apparent difference of mussel foot byssus, the secreted protein composition and distribution characteristics of mussel foot gland. Based on the apparent morphological differences of deep-sea mussels and sallow-sea mussels by SEM, two kind of mussels foot tissues are detected with confocal Raman micro-spectroscopy and acquire Raman spectra and 2D Raman color-coded image of three glands. The components and relative distribution of three glands of two mussels are compared and byssus differences caused by the glands distribution are analyzed. Meanwhile, considering the living environments of the two kind of mussels, we think that the mussel byssus appearance difference and foot glands distribution is a kind of adaptation mechanism to the environment. The Raman spectra suggest the components of two mussels foot glands: the higher intensity at 1 242 and 1 269 cm(-1) to the other two peaks (1 318 and 1 337 cm (-1)) of the amide III signals indicates that the core gland is a higher degree of conformational order protein. The proteins that comprise theplaque and cuticle enrich amino acid tyrosine (Tyr) at 643, 830, 850 and 1 615 cm(-1) and post-translationally converted to 3 ,4-dihydroxyphenylalanine (DOPA) at 785 cm(-1); shallow-sea mussels present high intensity collagen protein at 1 043 cm(-1). Raman imaging shows the glands distribution feature: deep-sea mussels foot glands distribution concentration and shallow-sea mussels foot glands distribution dispersion, which suggests that the glands distribution may be a kind of mechanism for the mussels to adapt different environments. The results indicate that confocal Raman micro-spectroscopy can be used in the analysis of the mussel foot glands distribution characteristics in different environments, and has great prospect to be applied in the micro-analysis of the biological samples.
KeywordConfocal Raman spectroscopy Mussel foot gland Adhesive protein
DOI10.3964/j.issn.1000-0593(2020)03-0755-05
Indexed BySCI
Language英语
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000528837900015
PublisherOFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167314
Collection海洋地质与环境重点实验室
Corresponding AuthorZhang Xin
Affiliation1.Chinese Acad Sci, CAS Key Lab Marine Geol & Environm, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao 266061, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liang Zheng-wei,Du Zeng-feng,Li Chao-lun,et al. Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2020,40(3):755-759.
APA Liang Zheng-wei.,Du Zeng-feng.,Li Chao-lun.,Wang Min-xiao.,Wang Bing.,...&Yan Jun.(2020).Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot.SPECTROSCOPY AND SPECTRAL ANALYSIS,40(3),755-759.
MLA Liang Zheng-wei,et al."Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot".SPECTROSCOPY AND SPECTRAL ANALYSIS 40.3(2020):755-759.
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