IOCAS-IR  > 海洋生态与环境科学重点实验室
Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis
Li, Xiao-dong1,2,3; Yan, Tian2,3,4; Zhang, Qing-chun2,3,4; Yu, Ren-cheng2,3,4; Zhou, Ming-jiang2
2020-05-01
Source PublicationMARINE ENVIRONMENTAL RESEARCH
ISSN0141-1136
Volume157Pages:8
Corresponding AuthorLi, Xiao-dong(lixiaodong11@163.com) ; Yan, Tian(tianyan@qdio.ac.cn)
AbstractBlooms of the dinoflagellate Karenia mikimotoi have cause great financial losses to the marine aquaculture industry. However, the toxicity mechanism of this species is still not fully known. In this study, we evaluated the short-term effects of K. mikimotoi on the rotifer Brachionus plicatilis by micro and sub micro observing and by measuring inhibition of crucial enzymes. Behaviour disorder, mucus production, corona and cilium damage, vesical production, and body shrinkage occurred within 1 h after rotifers were treated with K. mikimotoi at a density of 3 x 10(4) cells/mL. Enzyme activity assays showed that K. mikimotoi at low densities significantly inhibited multiple enzymes within 3 h, and obvious density-effect trends were also observed. For instance, activity of esterase and acetylcholinesterase of rotifers significantly decreased to 94.3/83.3% and 82.8/66.9% of control treatment values in 30 and 1000 cells/mL algal treatment, respectively. Total ATPase and Na+-K+-ATPase activities of rotifers also decreased to 82.3% and 68.6% of control values in 1000 cells/mL treatment. The LDH releasement test and MDA tests showed no significant difference between algae treatment and control. It suggested that K. mikimotoi might not cause significant cytolysis and oxidative damage to rotifers, but may cause mortality by inhibiting the activity of crucial enzymes, which may lead to cell permeability disorder and body shrinkage.
KeywordKarenia mikimotoi Brachionus plicatilis Enzymes inhibition Toxicity mechanism
DOI10.1016/j.marenvres.2019.104866
Indexed BySCI
Language英语
Funding ProjectScience & Technology Basic Resources Investigation Program of China[2018FY100206] ; National Nature Science Foundation of China[NSFC 41476102] ; National Nature Science Foundation of China[U1406403] ; National Marine Laboratory Key Program[2015ASKJ02] ; Sino-Australian Centre for Healthy Coasts[2016YFE0101500]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology ; Toxicology
WOS IDWOS:000528613500007
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167311
Collection海洋生态与环境科学重点实验室
Corresponding AuthorLi, Xiao-dong; Yan, Tian
Affiliation1.Fujian Agr & Forestry Univ, Coll Anim Sci, Inst Oceanol, Key Lab Marine Biotechnol Fujian Prov, Fuzhou 350002, Fujian, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xiao-dong,Yan, Tian,Zhang, Qing-chun,et al. Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis[J]. MARINE ENVIRONMENTAL RESEARCH,2020,157:8.
APA Li, Xiao-dong,Yan, Tian,Zhang, Qing-chun,Yu, Ren-cheng,&Zhou, Ming-jiang.(2020).Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis.MARINE ENVIRONMENTAL RESEARCH,157,8.
MLA Li, Xiao-dong,et al."Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis".MARINE ENVIRONMENTAL RESEARCH 157(2020):8.
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