Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei | |
Wang, Yue1,2,3; Yu, Yang1,2,4 ; Li, Shihao1,2,4; Zhang, Xiaojun1,2,4; Xiang, Jianhai1,2,4; Li, Fuhua1,2,4,5
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| 2020-04-12 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| ISSN | 1436-2228 |
| 页码 | 10 |
| 通讯作者 | Yu, Yang(yuyang@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn) |
| 摘要 | In order to learn the sex determination and differentiation process in shrimp, we performed sex-specific transcriptome sequencing of the Zoea I larvae in Litopenaeus vannamei (L. vannamei) using a DNA/RNA co-extraction method. The sex-specific genes and the sex-linked SNPs were identified. De novo assembly of all the clean reads generated 41,270 unigenes with an average length of 1026 bp and an N50 of 1922 bp. A total of 60 differentially expressed genes (DEGs) between females and males at Zoea I stage were obtained, in which 41 unigenes had annotations. DEGs that might be related to sex development in L. vannamei were analyzed. Besides, paralogous genes of some known sex-related genes in model organisms such as sex-lethal (Sxl) showed no sex-biased difference, which indicated they may not play roles in sex development at Zoea I stage. Candidate sex-linked SNPs were validated in six populations of L. vannamei, and SNPs in Unigene0020898 and Unigene0020336 were fully linked to genders, suggesting the two genes located in the sex-determining region of L. vannamei. Our report provided the sex-specific gene expression in early developmental stage of L. vannamei, and found some sex-biased genes that may participate in the sex development process. We also detected two sex-linked genes which may be located in the sex determination region of L. vannamei through bulked segregant analysis, and the SNPs on these genes also provided a method for genetic sex identification at transcriptional level. Our findings will facilitate further researches on molecular mechanism of sex determination and differentiation in shrimps. |
| 关键词 | Sex-specific genes Transcriptome sequencing Sex-linked genes Litopenaeus vannamei |
| DOI | 10.1007/s10126-020-09962-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Joint NSFC-ISF Research Program[3181101109] ; National Key R&D Program of China[2018YFD0901301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; China Agriculture Research System[48] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000525337900001 |
| 出版者 | SPRINGER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167206 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Yang; Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Wang, Yue,Yu, Yang,Li, Shihao,et al. Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei[J]. MARINE BIOTECHNOLOGY,2020:10. |
| APA | Wang, Yue,Yu, Yang,Li, Shihao,Zhang, Xiaojun,Xiang, Jianhai,&Li, Fuhua.(2020).Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei.MARINE BIOTECHNOLOGY,10. |
| MLA | Wang, Yue,et al."Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei".MARINE BIOTECHNOLOGY (2020):10. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Sex-Specific Transcr（499KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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