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Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions
Sun, Mingzhe1,2; Li, Shihao1,2,3; Zhang, Xiaojun1,2,3; Xiang, Jianhai1,2; Li, Fuhua1,2,3
2020-07-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号108页码:12
通讯作者Li, Fuhua(fhli@qdio.ac.cn)
摘要Hemocytes in shrimp play important roles in innate immune responses against pathogens. Although three types of hemocytes including hyalinocytes, semi-granulocytes and granulocytes were identified based on their morphological characters in penaeid shrimp, knowledge about the molecular basis of their functions in the immunity is still very limited. In the present study, three subpopulations of hemocytes were firstly separated by Percoll gradient centrifugation, and their transcriptomes were analyzed. The data showed that significantly differential gene expression patterns existed in different types of hemocytes. The genes encoding phagocytic receptors, lectins and actin cytoskeleton involved in phagocytosis were highly expressed in hyalinocytes, while genes involved in the humoral immunity signaling pathways were highly expressed in semi-granulocytes, and genes encoding prophenoloxidase (proPO)-activating enzyme and serine proteases involved in proPO system activation were highly expressed in granulocytes. Further flow cytometry analysis indicated that hyalinocytes were the main hemocytes subpopulation responsible for ingesting foreign fluorescent beads, and this ingestion process mainly depends on the endocytic way of macropinocytosis. These data provide valuable information for understanding the molecular basis of distinct shrimp hemocytes subpopulations of shrimp in cellular and humoral immunity.
关键词Hemocytes subpopulation Transcriptome Phagocytosis Macropinocytosis Penaeid shrimp
DOI10.1016/j.dci.2020.103689
收录类别SCI
语种英语
资助项目Major Science and Technology Innovation Projects in Shandong Province[2018SDKJ0302-5] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48[CARS-48]
WOS研究方向Fisheries ; Immunology ; Zoology
WOS类目Fisheries ; Immunology ; Zoology
WOS记录号WOS:000527338800013
出版者ELSEVIER SCI LTD
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167191
专题实验海洋生物学重点实验室
通讯作者Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室;  中国科学院海洋大科学研究中心
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GB/T 7714
Sun, Mingzhe,Li, Shihao,Zhang, Xiaojun,et al. Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,108:12.
APA Sun, Mingzhe,Li, Shihao,Zhang, Xiaojun,Xiang, Jianhai,&Li, Fuhua.(2020).Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,108,12.
MLA Sun, Mingzhe,et al."Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 108(2020):12.
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