| Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas | |
| Guevelou, Eric1; Huvet, Arnaud1; Sussarellu, Rossana1,2; Milan, Massimo3; Guo, Ximing4; Li, Li5; Zhang, Guofan5; Quillien, Virgile1; Daniel, Jean-Yves1; Quere, Claudie1; Boudry, Pierre1; Corporeau, Charlotte1; Corporeau, C | |
| 2013-07-01 | |
| 发表期刊 | JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
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| ISSN | 0174-1578 |
| 卷号 | 183期号:5页码:597-611 |
| 文章类型 | Article |
| 摘要 | AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.; AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia. |
| 关键词 | Marine Bivalve Crassostrea Gigas Hypoxia Amp-activated Protein Kinase Alternative Splicing Adductor Muscle |
| 学科领域 | Physiology ; Zoology |
| DOI | 10.1007/s00360-013-0743-6 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Physiology ; Zoology |
| WOS类目 | Physiology ; Zoology |
| WOS记录号 | WOS:000320733500002 |
| WOS关键词 | METABOLIC-RATE DEPRESSION ; RAT SKELETAL-MUSCLE ; GLUCOSE-TRANSPORT ; REPRODUCTIVE-CYCLE ; MOLECULAR-CLONING ; OOCYTE MATURATION ; CELLULAR-ENERGY ; GENE-EXPRESSION ; SUBUNIT GENE ; AKT ACTIVITY |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16717 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Corporeau, C |
| 作者单位 | 1.IFREMER, Ctr Bretagne ZI Pointe Diable, LEMAR, UMR 6539, F-29280 Plouzane, France 2.CNRS, IUEM UBO, UMR 6539, LEMAR, F-29280 Plouzane, France 3.Univ Padua, Dept Comparat Biomed & Food Sci, Legnaro, Italy 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guevelou, Eric,Huvet, Arnaud,Sussarellu, Rossana,et al. Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas[J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2013,183(5):597-611. |
| APA | Guevelou, Eric.,Huvet, Arnaud.,Sussarellu, Rossana.,Milan, Massimo.,Guo, Ximing.,...&Corporeau, C.(2013).Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,183(5),597-611. |
| MLA | Guevelou, Eric,et al."Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 183.5(2013):597-611. |
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| Regulation of a trun（967KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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