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Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS
Wang Baojie1,2; Liu Mei1; Jiang Keyong1; Zhang Guofan1; Wang Lei1; Wang, L
2013-03-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号31期号:2页码:407-415
文章类型Article
摘要Peptides in shrimp hemolymph play an important role in the innate immune response. Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection. We used magnetic bead-based purification (ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to characterize shrimp hemolymph peptides. Shrimp serum and plasma were used as the source of samples for comparative analysis, and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis. To screen potential specific biomarkers in serum of immune-challenged shrimps, we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps. The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software. Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide (LPS)-infection. The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%. Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph, and will help to enable a better understanding of the innate immune response of shrimps.; Peptides in shrimp hemolymph play an important role in the innate immune response. Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection. We used magnetic bead-based purification (ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to characterize shrimp hemolymph peptides. Shrimp serum and plasma were used as the source of samples for comparative analysis, and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis. To screen potential specific biomarkers in serum of immune-challenged shrimps, we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps. The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software. Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide (LPS)-infection. The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%. Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph, and will help to enable a better understanding of the innate immune response of shrimps.
关键词Fenneropenaeus Chinensis Hemolymph Peptidome Maldi-tof Ms Wcx-magnetic Bead Immune-challenged
学科领域Marine & Freshwater Biology ; Oceanography
DOI10.1007/s00343-013-2076-4
URL查看原文
收录类别SCI
语种英语
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS类目Limnology ; Oceanography
WOS记录号WOS:000316018300017
WOS关键词FLIGHT MASS-SPECTROMETRY ; MOLECULAR-CLONING ; ANTIMICROBIAL PEPTIDE ; LITOPENAEUS-VANNAMEI ; PROTEOMIC ANALYSIS ; PENAEUS-MONODON ; IMMUNE-RESPONSE ; NATURAL LECTIN ; PURIFICATION ; EXPRESSION
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16713
专题海洋生物技术研发中心
通讯作者Wang, L
作者单位1.Chinese Acad Sci, Inst Oceanl, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Wang Baojie,Liu Mei,Jiang Keyong,et al. Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):407-415.
APA Wang Baojie,Liu Mei,Jiang Keyong,Zhang Guofan,Wang Lei,&Wang, L.(2013).Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),407-415.
MLA Wang Baojie,et al."Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):407-415.
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