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Identification, functional characterization and the potential role of variable lymphocyte receptor EsVLRA from Eriocheir sinensis in response to secondary challenge after Vibrio parahaemolyticus vaccine
Liu, Hourong1,4; Song, Chengwen5; Ning, Junhao1,4; Liu, Yuan1; Cui, Zhaoxia2,3
2020-03-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号98页码:201-209
通讯作者Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
摘要Variable lymphocyte receptors (VLRs) play an important role via their antigen-special reorganization in jawless vertebrates (agnathans) adaptive immune response. In the present study, the open reading frame (ORF) of Eriocheir sinensis VLRA (designated as EsVLRA) was identified. EsVLRA comprised a 799-amino-acid polypeptide with one LRR_NT domain, thirteen LRR domains and one LRR_CT domain, which showed a high domain consistency of the VLR genes in lamprey (Petromyzon marinas). The transcript of EsVLRA was detected in all examined tissues with the highest level detected in hepatopancreas. Notably, the expression of EsVLRA in hepatopancreas, gonads, gill and intestine of male crabs was significantly higher than that in females. The recombinant EsVLRA exhibited strong bacteria-binding activity rather than antibacterial activity, suggesting its crucial role in immune recognition. Furthermore, 6 h earlier response and a significantly higher peak of EsVLRA mRNA expression was observed after challenge with live Vibrio parahaemolyticus (240.6-fold, P < 0.01, crabs receive secondary challenge after V. parahaemolyticus vaccine to the carbs only receive twice PBS injection, N = 6), compared with those only received first injection with formalin-inactivated V. parahaemolyticus (39.7-fold, P < 0.01, challenge 6 h to vaccination 12 h). The findings of this study together demonstrated that EsVLRA plays an important role in the immune system of E. sinensis, serving as a pattern recognition receptor and involving in the immune priming.
关键词Eriocheir sinensis Variable lymphocyte receptors Bacteria-binding activity Immune priming
DOI10.1016/j.fsi.2020.01.011
收录类别SCI
语种英语
资助项目National Key R&D Program of China[2018YFD0900303] ; Ten Thousand Talents Program[Y82344103H]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000516880900023
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167112
专题实验海洋生物学重点实验室
通讯作者Cui, Zhaoxia
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Huaiyin Inst Technol, Sch Life Sci & Food Engn, Huaian 223003, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Liu, Hourong,Song, Chengwen,Ning, Junhao,et al. Identification, functional characterization and the potential role of variable lymphocyte receptor EsVLRA from Eriocheir sinensis in response to secondary challenge after Vibrio parahaemolyticus vaccine[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:201-209.
APA Liu, Hourong,Song, Chengwen,Ning, Junhao,Liu, Yuan,&Cui, Zhaoxia.(2020).Identification, functional characterization and the potential role of variable lymphocyte receptor EsVLRA from Eriocheir sinensis in response to secondary challenge after Vibrio parahaemolyticus vaccine.FISH & SHELLFISH IMMUNOLOGY,98,201-209.
MLA Liu, Hourong,et al."Identification, functional characterization and the potential role of variable lymphocyte receptor EsVLRA from Eriocheir sinensis in response to secondary challenge after Vibrio parahaemolyticus vaccine".FISH & SHELLFISH IMMUNOLOGY 98(2020):201-209.
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