Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2 | |
| Sun, Yan-ling1,2; Liu, Shuang1,2,3; Sun, Li1,2 | |
| 2020-04-15 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 520Pages:10 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) |
| Abstract | yy MicroRNAs (miRNAs) are small, non-coding endogenous RNAs that play critical roles in diverse cellular processes through regulating target gene expression. In the present study, we identified two Japanese flounder (Paralichthys olivaceus) miRNAs, i.e., pol-miR-7133 and pol-miR-3p-9227, and examined the functions of the miRNAs and their target gene in association with the infection of Streptococcus iniae. pol-miR-7133 and pol-miR-3p-9227 were identified as miRNAs downregulated during S. iniae infection. Both miRNAs targeted the gene of lysosome-associated membrane protein 2 (LAMP2) isoform 3 of flounder (named PoLAMP23) by interaction with the 3'-untranslated region (3'-UTR) of PoLAMP2-3. Both in vivo and in vitro studies showed that PoLAMP2-3 expression negatively correlated with pol-miR-7133 and pol-miR-3p-9227 expression. Since to date no study on fish LAMP2 had been documented, we investigated the biological property of PoLAMP2-3. We found that PoLAMP2-3 expression occurred in multiple tissues and was most abundant in liver. PoLAMP2-3 is a highly acidic transmembrane protein and was observed to localize in both lysosome and cellular membrane. In vitro studies showed that overexpression of pol-miR-7133 and pol-miR-3p-9227 or interference with PoLAMP2-3 expression significantly enhanced S. iniae infection in flounder cells. Consistently, in vivo studies showed that knockdown of pol-miR-7133 and pol-miR-3p-9227, or overexpression of PoLAMP2-3, inhibited S. iniae infection in flounder, whereas knockdown of PoLAMP2-3 in flounder facilitated S. iniae infection. Together these results revealed for the first time an important immune function of fish LAMP2 and indicated that pol-miR-7133 and pol-miR-3p-9227, via LAMP2, play a vital role in anti-bacterial immunity in fish. |
| Keyword | Streptococcus iniae Paralichthys olivaceus miRNA Lysosome-associated membrane protein Bacterial infection Immune defense |
| DOI | 10.1016/j.aquaculture.2020.734980 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31730100] ; National Natural Science Foundation of China[31902408] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000515498200073 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167109 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Sun, Yan-ling,Liu, Shuang,Sun, Li. pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2[J]. AQUACULTURE,2020,520:10. |
| APA | Sun, Yan-ling,Liu, Shuang,&Sun, Li.(2020).pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2.AQUACULTURE,520,10. |
| MLA | Sun, Yan-ling,et al."pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2".AQUACULTURE 520(2020):10. |
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| pol-miR-7133 and pol（1251KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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