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pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2
Sun, Yan-ling1,2; Liu, Shuang1,2,3; Sun, Li1,2
2020-04-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume520Pages:10
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
Abstractyy MicroRNAs (miRNAs) are small, non-coding endogenous RNAs that play critical roles in diverse cellular processes through regulating target gene expression. In the present study, we identified two Japanese flounder (Paralichthys olivaceus) miRNAs, i.e., pol-miR-7133 and pol-miR-3p-9227, and examined the functions of the miRNAs and their target gene in association with the infection of Streptococcus iniae. pol-miR-7133 and pol-miR-3p-9227 were identified as miRNAs downregulated during S. iniae infection. Both miRNAs targeted the gene of lysosome-associated membrane protein 2 (LAMP2) isoform 3 of flounder (named PoLAMP23) by interaction with the 3'-untranslated region (3'-UTR) of PoLAMP2-3. Both in vivo and in vitro studies showed that PoLAMP2-3 expression negatively correlated with pol-miR-7133 and pol-miR-3p-9227 expression. Since to date no study on fish LAMP2 had been documented, we investigated the biological property of PoLAMP2-3. We found that PoLAMP2-3 expression occurred in multiple tissues and was most abundant in liver. PoLAMP2-3 is a highly acidic transmembrane protein and was observed to localize in both lysosome and cellular membrane. In vitro studies showed that overexpression of pol-miR-7133 and pol-miR-3p-9227 or interference with PoLAMP2-3 expression significantly enhanced S. iniae infection in flounder cells. Consistently, in vivo studies showed that knockdown of pol-miR-7133 and pol-miR-3p-9227, or overexpression of PoLAMP2-3, inhibited S. iniae infection in flounder, whereas knockdown of PoLAMP2-3 in flounder facilitated S. iniae infection. Together these results revealed for the first time an important immune function of fish LAMP2 and indicated that pol-miR-7133 and pol-miR-3p-9227, via LAMP2, play a vital role in anti-bacterial immunity in fish.
KeywordStreptococcus iniae Paralichthys olivaceus miRNA Lysosome-associated membrane protein Bacterial infection Immune defense
DOI10.1016/j.aquaculture.2020.734980
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31730100] ; National Natural Science Foundation of China[31902408] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000515498200073
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167109
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Yan-ling,Liu, Shuang,Sun, Li. pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2[J]. AQUACULTURE,2020,520:10.
APA Sun, Yan-ling,Liu, Shuang,&Sun, Li.(2020).pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2.AQUACULTURE,520,10.
MLA Sun, Yan-ling,et al."pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2".AQUACULTURE 520(2020):10.
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