An acid-tolerance response system protecting exponentially growing Escherichia coli

doi:10.1038/s41467-020-15350-5

IOCAS-IR

	An acid-tolerance response system protecting exponentially growing Escherichia coli
	Xu, Ying 1,2; Zhao, Zhe 1,2; Tong, Wenhua 1,7; Ding, Yamei 3; Liu, Bin 4; Shi, Yixin 5; Wang, Jichao 1; Sun, Shenmei 1,2; Liu, Min 1; Wang, Yuhui 4; Qi, Qingsheng 6; Xian, Mo 1; Zhao, Guang 1,6
	2020-03-20
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	11 期号:1 页码:13
通讯作者	Xian, Mo(xianmo@qibebt.ac.cn) ; Zhao, Guang(zhaoguang@qibebt.ac.cn)
摘要	The ability to grow at moderate acidic conditions (pH 4.0-5.0) is important to Escherichia coli colonization of the host's intestine. Several regulatory systems are known to control acid resistance in E. coli, enabling the bacteria to survive under acidic conditions without growth. Here, we characterize an acid-tolerance response (ATR) system and its regulatory circuit, required for E. coli exponential growth at pH 4.2. A two-component system CpxRA directly senses acidification through protonation of CpxA periplasmic histidine residues, and upregulates the fabA and fabB genes, leading to increased production of unsaturated fatty acids. Changes in lipid composition decrease membrane fluidity, F0F1-ATPase activity, and improve intracellular pH homeostasis. The ATR system is important for E. coli survival in the mouse intestine and for production of higher level of 3-hydroxypropionate during fermentation. Furthermore, this ATR system appears to be conserved in other Gram-negative bacteria. The ability to grow at acidic pH is crucial for E. coli colonization of the host's intestine. Here, the authors identify an acid-tolerance response system that is important for E. coli exponential growth at pH 4.2, survival in the mouse intestine, and production of 3-hydroxypropionate during fermentation.
DOI	10.1038/s41467-020-15350-5
收录类别	SCI
语种	英语
资助项目	NSFC[31722001] ; NSFC[31670089] ; Natural Science Foundation of Shandong Province[JQ201707]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000522096100016
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：66[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167103
专题	中国科学院海洋研究所
通讯作者	Xian, Mo; Zhao, Guang
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China 5.Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 USA 6.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China 7.Sichuan Univ Sci & Engn, Yibin 644000, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Xu, Ying,Zhao, Zhe,Tong, Wenhua,et al. An acid-tolerance response system protecting exponentially growing Escherichia coli[J]. NATURE COMMUNICATIONS,2020,11(1):13.
APA	Xu, Ying.,Zhao, Zhe.,Tong, Wenhua.,Ding, Yamei.,Liu, Bin.,...&Zhao, Guang.(2020).An acid-tolerance response system protecting exponentially growing Escherichia coli.NATURE COMMUNICATIONS,11(1),13.
MLA	Xu, Ying,et al."An acid-tolerance response system protecting exponentially growing Escherichia coli".NATURE COMMUNICATIONS 11.1(2020):13.

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