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An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study
Li, Xiuxue1,2,3,4; Wang, Lijun1,2,3,4; Jia, Xiaoling1,2,3,4; Guo, Chuanlong1,2,3,4,5; Li, Chao1,2,3,4; Zhang, Jiajia1,2,3,4; Shi, Dayong1,2,3,4,6
2020-04-06
Source PublicationJOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
ISSN2093-3134
Volume11Issue:1Pages:7
Corresponding AuthorShi, Dayong(shidayong@sdu.edu.cn)
AbstractBackground Bromophenol-thiosemicarbazone hybrid was a novel synthetic brominated anticancer compound with two bromine atoms. Bromophenol-thiosemicarbazone hybrid showed considerable selective inhibitory activity against PARP1 (IC50 = 29.5 nmol/L). UHPLC-TOF-MS was used to establish a new method to quantify bromophenol-thiosemicarbazone hybrid bio-samples for indispensable quantitation analysis in further extensive pre-clinical studies. Methods Chromatographic and mass spectrometry parameters were optimized for quantitative method establishment. Improved protein-precipitated method was applied to the extraction of bromophenol-thiosemicarbazone hybrid in rat plasma samples. Furthermore, this proposed method was applied to an intravenous bolus dose to male rats. Results Mobile phase was consisted of water for A and acetonitrile for B with 25 mmol/L formic acid in both A and B. The flow rate was 0.30 mL/min, and the run time of bromophenol-thiosemicarbazone hybrid was 4.0 min. A Thermo Fisher Accucore 2.6 mu m C18 column (50 x 2.1 mm i.d.; San Jose, USA) was used for chromatographic separation. High resolution mass spectrometry was used to quantify samples by exact mass number of compound which was operated on negative ionization mode. Linear dynamic range of the established method was widely with 13.7-10000 nmol/L. Pharmacokinetics properties of bromophenol-thiosemicarbazone hybrid were shown in the results. Conclusion This method was reliable and reproducible from sample preparation to analysis and storage stability under the investigated conditions. It may be useful for analysis of halogenated compounds and brominated compounds in ultra-performance liquid chromatography-mass spectrometry.
KeywordNovel compound Quantitation UHPLC-TOF-MS Pharmacokinetics
DOI10.1186/s40543-020-00210-0
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81773586] ; National Natural Science Foundation of China[81872906] ; National Natural Science Foundation of China[81703354] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-DQC014] ; Shandong Provincial Natural Science Foundation for Distinguished Young Scholars[JQ201722] ; NSFC-Shandong Joint Fund Project[U1706213]
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000523574700001
PublisherSPRINGER INTERNATIONAL PUBLISHING AG
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167094
Collection实验海洋生物学重点实验室
Corresponding AuthorShi, Dayong
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Qingdao Univ Sci & Technol, Coll Chem Engn, Dept Pharm, Qingdao, Peoples R China
6.Shandong Univ, State Key Lab Microbial Technol, 72 Binhai Rd, Qingdao 250100, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xiuxue,Wang, Lijun,Jia, Xiaoling,et al. An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study[J]. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,2020,11(1):7.
APA Li, Xiuxue.,Wang, Lijun.,Jia, Xiaoling.,Guo, Chuanlong.,Li, Chao.,...&Shi, Dayong.(2020).An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study.JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,11(1),7.
MLA Li, Xiuxue,et al."An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study".JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY 11.1(2020):7.
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