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| An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study | |
| Li, Xiuxue1,2,3,4; Wang, Lijun1,2,3,4; Jia, Xiaoling1,2,3,4; Guo, Chuanlong1,2,3,4,5; Li, Chao1,2,3,4; Zhang, Jiajia1,2,3,4; Shi, Dayong1,2,3,4,6 | |
| 2020-04-06 | |
| Source Publication | JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
 |
| ISSN | 2093-3134 |
| Volume | 11Issue:1Pages:7 |
| Corresponding Author | Shi, Dayong(shidayong@sdu.edu.cn) |
| Abstract | Background Bromophenol-thiosemicarbazone hybrid was a novel synthetic brominated anticancer compound with two bromine atoms. Bromophenol-thiosemicarbazone hybrid showed considerable selective inhibitory activity against PARP1 (IC50 = 29.5 nmol/L). UHPLC-TOF-MS was used to establish a new method to quantify bromophenol-thiosemicarbazone hybrid bio-samples for indispensable quantitation analysis in further extensive pre-clinical studies. Methods Chromatographic and mass spectrometry parameters were optimized for quantitative method establishment. Improved protein-precipitated method was applied to the extraction of bromophenol-thiosemicarbazone hybrid in rat plasma samples. Furthermore, this proposed method was applied to an intravenous bolus dose to male rats. Results Mobile phase was consisted of water for A and acetonitrile for B with 25 mmol/L formic acid in both A and B. The flow rate was 0.30 mL/min, and the run time of bromophenol-thiosemicarbazone hybrid was 4.0 min. A Thermo Fisher Accucore 2.6 mu m C18 column (50 x 2.1 mm i.d.; San Jose, USA) was used for chromatographic separation. High resolution mass spectrometry was used to quantify samples by exact mass number of compound which was operated on negative ionization mode. Linear dynamic range of the established method was widely with 13.7-10000 nmol/L. Pharmacokinetics properties of bromophenol-thiosemicarbazone hybrid were shown in the results. Conclusion This method was reliable and reproducible from sample preparation to analysis and storage stability under the investigated conditions. It may be useful for analysis of halogenated compounds and brominated compounds in ultra-performance liquid chromatography-mass spectrometry. |
| Keyword | Novel compound Quantitation UHPLC-TOF-MS Pharmacokinetics |
| DOI | 10.1186/s40543-020-00210-0 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[81773586] ; National Natural Science Foundation of China[81872906] ; National Natural Science Foundation of China[81703354] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-DQC014] ; Shandong Provincial Natural Science Foundation for Distinguished Young Scholars[JQ201722] ; NSFC-Shandong Joint Fund Project[U1706213] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000523574700001 |
| Publisher | SPRINGER INTERNATIONAL PUBLISHING AG |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167094 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Shi, Dayong |
| Affiliation | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Qingdao Univ Sci & Technol, Coll Chem Engn, Dept Pharm, Qingdao, Peoples R China 6.Shandong Univ, State Key Lab Microbial Technol, 72 Binhai Rd, Qingdao 250100, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Xiuxue,Wang, Lijun,Jia, Xiaoling,et al. An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study[J]. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,2020,11(1):7. |
| APA | Li, Xiuxue.,Wang, Lijun.,Jia, Xiaoling.,Guo, Chuanlong.,Li, Chao.,...&Shi, Dayong.(2020).An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study.JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,11(1),7. |
| MLA | Li, Xiuxue,et al."An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study".JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY 11.1(2020):7. |
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