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Japanese flounder Paralichthys olivaceus interleukin 21 induces inflammatory response and plays a vital role in the immune defense against bacterial pathogen
Yu, Chao1,2,3; Zhang, Peng1,2,3; Li, Xue-peng1,2; Sun, Li1,2
2020-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume98Pages:364-373
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractInterleukin (IL)-21 is a pleiotropic cytokine and plays a vital role in immunity. In the current study, we examined the immune function of Japanese flounder Paralichthys olivaceus IL-21 (PoIL-21). PoIL-21 shares moderate (25.17%-46.25%) sequence identities with other teleost IL-21. PoIL-21 expression occurred in multiple tissues, especially intestine, and was regulated by bacterial infection in a time dependent manner. PoIL-21 was secreted by peripheral blood leukocytes (PBL) upon LPS stimulation. Recombinant PoIL-21 (rPoIL-21) bound to a wide range of Gram-negative and Gram-positive bacteria and inhibited the growth of the fish bacterial pathogen Streptococcus iniae. rPoIL-21 also interacted with PBL, resulting in enhanced cell proliferation, ROS production, and expression of IL-1 beta, TNF-alpha, CD8 beta, T-bet, PoIL-21, PoIL-21 receptor, and STAT. Consequently, the presence of rPoIL-21 significantly reduced bacterial infection in PBL. In vivo study showed that rPoIL-21 upregulated the expression of inflammatory cytokines and PoIL-21. Taken together, these results indicate that PoIL-21 is an inducible, secreted cytokine with a broad range of binding capacities and plays an important role in the regulation of anti-bacterial immunity.
KeywordInterleukin-21 Paralichthys olivaceus Inflammatory cytokines T cell activation Immune defense Bacterial infection
DOI10.1016/j.fsi.2020.01.043
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key Research & Development Project of China[2018YFD0900500] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000516880900041
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167091
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yu, Chao,Zhang, Peng,Li, Xue-peng,et al. Japanese flounder Paralichthys olivaceus interleukin 21 induces inflammatory response and plays a vital role in the immune defense against bacterial pathogen[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:364-373.
APA Yu, Chao,Zhang, Peng,Li, Xue-peng,&Sun, Li.(2020).Japanese flounder Paralichthys olivaceus interleukin 21 induces inflammatory response and plays a vital role in the immune defense against bacterial pathogen.FISH & SHELLFISH IMMUNOLOGY,98,364-373.
MLA Yu, Chao,et al."Japanese flounder Paralichthys olivaceus interleukin 21 induces inflammatory response and plays a vital role in the immune defense against bacterial pathogen".FISH & SHELLFISH IMMUNOLOGY 98(2020):364-373.
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