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| Characterization of Streptococcus iniae-induced microRNA profiles in Paralichthys olivaceus and identification of pol-3p-10740_175 as a regulator of antibacterial immune response | |
Liu, Shuang1,2,3; Ning, Xian-hui1,2; Guan, Xiao-lu1,2,3; Li, Xue-peng1,2 ; Sun, Li1,2
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| 2020-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 98页码:860-867 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 摘要 | MicroRNAs (miRNAs) are involved in many biological activities including immune defense against pathogens. In this study, we applied high-throughput sequencing technology to examine miRNAs in Japanese flounder (Paralichthys olivaceus) infected with Streptococcus iniae at different times. A total of 1038 miRNAs were identified, of which, 249 were novel miRNAs, and 81 showed differential expression (named DEmiRNAs) after S. iniae infection. Of the 81 DEmiRNAs identified, 34 and 58 occurred at 6 h and 24 h post-infection, respectively; most DEmiRNAs were strongly time-specific, and only 13.6% of the DEmiRNAs were shared between the two time points. A total of 9582 target genes were predicted for the 81 DEmiRNAs. The putative target genes were enriched in various GO and KEGG pathways of biological processes and molecular/cellular functions, in particular endocytosis, regulation of transcription, lysososme, and the signaling pathways of MAPK, ErbB, and AMPK. One of the DEmiRNAs, poi-3p-10740_175, was found to target dual specificity phosphatase 6 (Dusp6) and repress the expression of the latter. Transfection of flounder FG cells with pol-3p-10740_175 caused a significant inhibition on S. iniae invasion. The results of this study provided the first S. iniae-induced miRNA profile in Japanese flounder and indicated that flounder miRNAs play an important role in antibacterial immunity. |
| 关键词 | Streptococcus iniae Paralichthys olivaceus miRNA High-throughput sequencing Immune response |
| DOI | 10.1016/j.fsi.2019.11.045 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; National Key Research & Development Project of China[2018YFD0900500] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000516880900090 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167081 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Liu, Shuang,Ning, Xian-hui,Guan, Xiao-lu,et al. Characterization of Streptococcus iniae-induced microRNA profiles in Paralichthys olivaceus and identification of pol-3p-10740_175 as a regulator of antibacterial immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:860-867. |
| APA | Liu, Shuang,Ning, Xian-hui,Guan, Xiao-lu,Li, Xue-peng,&Sun, Li.(2020).Characterization of Streptococcus iniae-induced microRNA profiles in Paralichthys olivaceus and identification of pol-3p-10740_175 as a regulator of antibacterial immune response.FISH & SHELLFISH IMMUNOLOGY,98,860-867. |
| MLA | Liu, Shuang,et al."Characterization of Streptococcus iniae-induced microRNA profiles in Paralichthys olivaceus and identification of pol-3p-10740_175 as a regulator of antibacterial immune response".FISH & SHELLFISH IMMUNOLOGY 98(2020):860-867. |
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