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Growth, stress and non-specific immune responses of turbot (Scophthalmus maximus) larvae exposed to different light spectra
Wu, Lele1,2,3,4; Wang, Yunong1,2,3,4; Han, Mingming1,2,3; Song, Zongcheng5; Song, Changbin6; Xu, Shihong1,2,3; Li, Jun1,2,3; Wang, Yanfeng1,2,3; Li, Xian1,2,3; Yue, Xinlu5
2020-04-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume520Pages:9
Corresponding AuthorLi, Jun(junli@qdio.ac.cn) ; Li, Xian(lixian@qdio.ac.cn)
AbstractThe welfare of farmed fish is an important issue in aquaculture. There is particular concern regarding ambient light in culture systems. In the current study, turbot (Scophthalmus maximus) larvae were exposed to four different light spectra: full spectrum (LDF), blue (LDB, peak at 450 nm), orange (LDO, peak at 595 nm) and red (LDR, peak at 629 nm) from the embryo stage to 60 days post hatching (dph). During this time, the growth and immune response of the turbot were evaluated. Whole fish tissue samples were collected and weighed. mRNA expression levels of insulin-like growth factor-I (IGF-I), heat shock protein 70 (HSP70), glutathione S-transferase (GST), catalase (CAT), metallothionein (MT), lysozyme (LZM), cathepsin D (CTSD), cathepsin F (CTSF) and the enzyme activity of superoxide dismutase (SOD), CAT, and peroxidase (POD) were measured at 10, 20, 30, 40, 50, and 60 dph. The results showed that LDB had a positive effect on the growth of turbot larvae at 20, 40, 50 and 60 dph, while this effect was not significant at 10 and 30 dph. Levels of IGF-I mRNA also showed a similar tendency, indicating the crucial interaction between IGF-I and somatic growth. The effect of light spectrum on the stress response of turbot larvae was stage specific. At 10 dph, prior to metamorphosis, long wavelengths could induce a stress response in turbot larvae. At 20 dph, metamorphosis began and no significant stress response was observed in larvae exposed to different light spectra; however, LDO significantly up-regulated SOD, CAT, POD and LZM activity. Metamorphosis continued until 56 dph. From 20 to 60 dph long wavelengths (LDR and LDO) resulted in a stress response characterized by high mRNA expression levels of HSP70, GST, CAT, MT, LZM, CTSD and CTSF. SOD, CAT, POD and LZM activities were inhibited, indicating increased enzyme consumption to maintain homeostasis. Overall, our results suggested that LDF should be used in turbot culture systems prior to metamorphosis and LDB should be used after metamorphosis. These findings are in keeping with the changes in light spectra experienced by turbot larvae undergoing metamorphosis in the wild.
KeywordScophthalmus maximus Light spectrum Growth Non-specific immunity Larvae
DOI10.1016/j.aquaculture.2020.734950
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFD0404000] ; Key R&D Program of Shandong Province[2018GHY115031] ; National Natural Science Foundation[41976122] ; Key R&D Program of Guangdong Province[2019B020215001] ; China Agriculture Research System[CARS-47]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000515498200048
PublisherELSEVIER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167078
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Jun; Li, Xian
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
6.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Lele,Wang, Yunong,Han, Mingming,et al. Growth, stress and non-specific immune responses of turbot (Scophthalmus maximus) larvae exposed to different light spectra[J]. AQUACULTURE,2020,520:9.
APA Wu, Lele.,Wang, Yunong.,Han, Mingming.,Song, Zongcheng.,Song, Changbin.,...&Yue, Xinlu.(2020).Growth, stress and non-specific immune responses of turbot (Scophthalmus maximus) larvae exposed to different light spectra.AQUACULTURE,520,9.
MLA Wu, Lele,et al."Growth, stress and non-specific immune responses of turbot (Scophthalmus maximus) larvae exposed to different light spectra".AQUACULTURE 520(2020):9.
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