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Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris
Li, Rongfeng1; Yu, Huahua1; Xing, Ronge1; Liu, Song1; Qing, Yukun1; Li, Kecheng1,2; Li, Bing1,2; Meng, Xiangtao1,2; Cui, Jinhui1,2; Li, Pengcheng1; Yu, HH
2013-09-01
发表期刊TOXICOLOGY IN VITRO
ISSN0887-2333
卷号27期号:6页码:1620-1625
文章类型Article
摘要Jellyfish venom contains various toxins and can cause itching, edema, muscle aches, shortness of breath, blood pressure depression, shock or even death after being stung. Hemolytic protein is one of the most hazardous components in the venom. The present study investigated the hemolytic activity of the nematocyst venom from jellyfish Stomolophus meleagris. Anion exchange chromatography, DEAE Sepharose Fast Flow, and gel filtration chromatography, Superdex200 had been employed to isolate hemolytic proteins from the nematocyst venom of jellyfish S. meleagris. Hemolysis of chicken red blood cells was used to quantify hemolytic potency of crude nematocyst venom and chromatography fractions during the purification process. Native-PAGE profile displayed one protein band in the purified hemolytic protein (SmTX); however, two protein bands with apparent molecular weights of similar to 45 kDa and 52 kDa were observed in the reducing SDS-PAGE analysis. Approximately 70 mu g/mL of SmTX caused 50% hemolysis (HU50) of the erythrocyte suspension. The hemolytic activity of SmTX was shown to be temperature and pH dependent, with the optimum temperature and pH being 37 degrees C and pH 5.0. The present study is the first report of isolation and partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish S. meleagris. The mechanism of the hemolytic activity of SmTX is not clear and deserves further investigation. (C) 2013 Published by Elsevier Ltd.; Jellyfish venom contains various toxins and can cause itching, edema, muscle aches, shortness of breath, blood pressure depression, shock or even death after being stung. Hemolytic protein is one of the most hazardous components in the venom. The present study investigated the hemolytic activity of the nematocyst venom from jellyfish Stomolophus meleagris. Anion exchange chromatography, DEAE Sepharose Fast Flow, and gel filtration chromatography, Superdex200 had been employed to isolate hemolytic proteins from the nematocyst venom of jellyfish S. meleagris. Hemolysis of chicken red blood cells was used to quantify hemolytic potency of crude nematocyst venom and chromatography fractions during the purification process. Native-PAGE profile displayed one protein band in the purified hemolytic protein (SmTX); however, two protein bands with apparent molecular weights of similar to 45 kDa and 52 kDa were observed in the reducing SDS-PAGE analysis. Approximately 70 mu g/mL of SmTX caused 50% hemolysis (HU50) of the erythrocyte suspension. The hemolytic activity of SmTX was shown to be temperature and pH dependent, with the optimum temperature and pH being 37 degrees C and pH 5.0. The present study is the first report of isolation and partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish S. meleagris. The mechanism of the hemolytic activity of SmTX is not clear and deserves further investigation. (C) 2013 Published by Elsevier Ltd.
关键词Jellyfish Stomolophus Meleagris Nematocyst Venom Isolation Hemolysis Smtx
学科领域Toxicology
DOI10.1016/j.tiv.2013.04.004
URL查看原文
收录类别SCI
语种英语
WOS研究方向Toxicology
WOS类目Toxicology
WOS记录号WOS:000324847800003
WOS关键词RHOPILEMA-ESCULENTUM KISHINOUYE ; CYANEA-NOZAKII KISHINOUYE ; BOX JELLYFISH ; PARTIAL-PURIFICATION ; SEA WASP ; CARYBDEA-MARSUPIALIS ; CHIRONEX-FLECKERI ; TOXIN ; BINDING ; ALATA
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16706
专题海洋生物技术研发中心
海洋环境工程技术研究发展中心
通讯作者Yu, HH
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris[J]. TOXICOLOGY IN VITRO,2013,27(6):1620-1625.
APA Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Yu, HH.(2013).Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris.TOXICOLOGY IN VITRO,27(6),1620-1625.
MLA Li, Rongfeng,et al."Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris".TOXICOLOGY IN VITRO 27.6(2013):1620-1625.
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