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Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
Liu, Youli1,2,3; Zhu, Qihui1,4; Li, Li1,2,5,6; Wang, Wei1,5,6,7; Zhang, Guofan1,5,6,7
2020-04-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume22Issue:2Pages:167-179
Corresponding AuthorLi, Li(lili@qdio.ac.cn)
AbstractThe Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster.
KeywordHSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq
DOI10.1007/s10126-019-09942-6
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000520822500002
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167063
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
7.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Youli,Zhu, Qihui,Li, Li,et al. Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq[J]. MARINE BIOTECHNOLOGY,2020,22(2):167-179.
APA Liu, Youli,Zhu, Qihui,Li, Li,Wang, Wei,&Zhang, Guofan.(2020).Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq.MARINE BIOTECHNOLOGY,22(2),167-179.
MLA Liu, Youli,et al."Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq".MARINE BIOTECHNOLOGY 22.2(2020):167-179.
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