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TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis
Wang, Di1,3; Zhang, Shujing1; Liu, Baozhong1,2,3
2020-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume98Pages:1017-1023
Corresponding AuthorLiu, Baozhong(bzliu@qdio.ac.cn)
AbstractTAF5L is a component of the P300/CBP-associated factor (PCAF) histone acetylase complex, which serves as a coactivator and takes part in basal transcription such as promoter recognition, complex assembly and transcription initiation. In our study, the full-length sequence of MpTAF5L was identified and characterized in the clam M. petechialis. Sequence analysis showed that the predicted MpTAF5L protein had a N-terminal TAF5-NTD2 domain and a C-terminal WD40-repeats domain. The annotation and evolutionary analysis revealed MpTAF5L had close evolutionary relationship with other invertebrate species. Tissue distribution analysis of TAFSL claimed that it was highly expressed in the mantle, adductor muscle, foot and hepatopancreas. The mRNA expression of MpTAF5L was significantly up-regulated after Vibrio parahaemolyticus challenge, indicating its involvement in the immune response of clam. Yeast two-hybrid assays verified that MpTAF5L can interact with MpMITF (a critical immune-related transcription factor), and our further research clarified this interaction depended upon the N-terminal TAF5-NTD2 domain of MpTAF5L. Moreover, the mRNA expression of MpBcl-2 (a target gene of MITF) was significantly decreased but the mRNA expression of MpMITF was not significantly changed after knockdown of MpTAF5L, which indicated the reduction of MpMITF regulating activity at the same time. These results revealed that MpTAF5L interacted with MpMITF and enhanced the activation of MpMITF, which plays roles in the immune defense against V. parahaemolyticus.
KeywordTAF5L MITF Innate immunity Meretrix petechialis
DOI10.1016/j.fsi.2019.11.039
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; China Agriculture Research System[CARS-49] ; National Natural Science Foundation of China[31772845]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000516880900107
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167042
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Baozhong
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Di,Zhang, Shujing,Liu, Baozhong. TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:1017-1023.
APA Wang, Di,Zhang, Shujing,&Liu, Baozhong.(2020).TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,98,1017-1023.
MLA Wang, Di,et al."TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 98(2020):1017-1023.
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