Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis | |
Wang, Di1,3; Zhang, Shujing1; Liu, Baozhong1,2,3
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| 2020-03-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 98Pages:1017-1023 |
| Corresponding Author | Liu, Baozhong(bzliu@qdio.ac.cn) |
| Abstract | TAF5L is a component of the P300/CBP-associated factor (PCAF) histone acetylase complex, which serves as a coactivator and takes part in basal transcription such as promoter recognition, complex assembly and transcription initiation. In our study, the full-length sequence of MpTAF5L was identified and characterized in the clam M. petechialis. Sequence analysis showed that the predicted MpTAF5L protein had a N-terminal TAF5-NTD2 domain and a C-terminal WD40-repeats domain. The annotation and evolutionary analysis revealed MpTAF5L had close evolutionary relationship with other invertebrate species. Tissue distribution analysis of TAFSL claimed that it was highly expressed in the mantle, adductor muscle, foot and hepatopancreas. The mRNA expression of MpTAF5L was significantly up-regulated after Vibrio parahaemolyticus challenge, indicating its involvement in the immune response of clam. Yeast two-hybrid assays verified that MpTAF5L can interact with MpMITF (a critical immune-related transcription factor), and our further research clarified this interaction depended upon the N-terminal TAF5-NTD2 domain of MpTAF5L. Moreover, the mRNA expression of MpBcl-2 (a target gene of MITF) was significantly decreased but the mRNA expression of MpMITF was not significantly changed after knockdown of MpTAF5L, which indicated the reduction of MpMITF regulating activity at the same time. These results revealed that MpTAF5L interacted with MpMITF and enhanced the activation of MpMITF, which plays roles in the immune defense against V. parahaemolyticus. |
| Keyword | TAF5L MITF Innate immunity Meretrix petechialis |
| DOI | 10.1016/j.fsi.2019.11.039 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; China Agriculture Research System[CARS-49] ; National Natural Science Foundation of China[31772845] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000516880900107 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167042 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Baozhong |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Di,Zhang, Shujing,Liu, Baozhong. TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:1017-1023. |
| APA | Wang, Di,Zhang, Shujing,&Liu, Baozhong.(2020).TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,98,1017-1023. |
| MLA | Wang, Di,et al."TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 98(2020):1017-1023. |
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| TAF5L functions as t（2246KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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