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High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography
Li, Kecheng1,2; Liu, Song1; Xing, Ronge1; Yu, Huahua1; Qin, Yukun1; Li, Rongfeng1; Li, Pengcheng1; Li, PC
2013-04-01
发表期刊JOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
卷号36期号:7页码:1275-1282
文章类型Article
摘要Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R-2 = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system.; Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R-2 = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system.
关键词Chitooligomers Chitosan Ion-exchange Chromatography
学科领域Chemistry
DOI10.1002/jssc.201200935
URL查看原文
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000319865700016
WOS关键词MOLECULAR-WEIGHT ; LIQUID-CHROMATOGRAPHY ; BIOLOGICAL-ACTIVITIES ; HYDROGEN-PEROXIDE ; CHITOSAN ; OLIGOSACCHARIDES ; CHITOOLIGOSACCHARIDES ; OLIGOMERS ; DEGRADATION ; MEDICINE
WOS标题词Science & Technology ; Physical Sciences
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16703
专题海洋生物技术研发中心
海洋环境工程技术研究发展中心
通讯作者Li, PC
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所
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Li, Kecheng,Liu, Song,Xing, Ronge,et al. High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(7):1275-1282.
APA Li, Kecheng.,Liu, Song.,Xing, Ronge.,Yu, Huahua.,Qin, Yukun.,...&Li, PC.(2013).High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.JOURNAL OF SEPARATION SCIENCE,36(7),1275-1282.
MLA Li, Kecheng,et al."High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography".JOURNAL OF SEPARATION SCIENCE 36.7(2013):1275-1282.
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