Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)

doi:10.1016/j.theriogenology.2013.06.016

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	Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)
	Lin, F.1,2; Zhao, C. Y.1,2; Xu, S. H.1; Ma, D. Y.1; Xiao, Z. Z.1; Xiao, Y. S.1; Xu, C. A.3; Liu, Q. H.1; Li, J.1 ; Li, J
	2013-10-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	80 期号:6 页码:665-672
文章类型	Article
摘要	Germ cells are indispensable for gonadal development and fertility. However, the physiological mechanisms regulating germ cell development in marine fish are poorly understood due to a lack of germ cell markers. The dead end (dnd) gene is a vertebrate-specific component of germplasm crucial for primordial germ cells (PGCs) migration and development in teleosts. In this study, we identified a dnd homologue (Smdnd) in turbot (Scophthalmus maximus) and investigated its expression pattern during embryogenesis and gonadal development. The deduced amino acid sequence of Smdnd shared several conserved motifs of Dnd homologues as well as high identity to other Dnd proteins. Phylogenetic analysis revealed that the SmDnd was closely related to its teleost counterparts. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization revealed that Smdnd transcripts could be exclusively detected in germ cells, including presumptive PGC and adult male and female germ cells. In addition, an interesting sexually dimorphic expression of Smdnd during gonadal development was observed by real-time PCR. Female turbot showed greater (P < 0.05) Smdnd expression than male before sex maturation. This difference reduced gradually due to the upregulation of Smdnd in the male during the period corresponding to spermatogonia proliferation and meiosis. These results indicate that Smdnd can be used as a germ cell marker in turbot. In addition, the temporal and sex differences in Smdnd expression indicate that this gene may play different roles in gonadal development in both sexes. (C) 2013 Elsevier Inc. All rights reserved.; Germ cells are indispensable for gonadal development and fertility. However, the physiological mechanisms regulating germ cell development in marine fish are poorly understood due to a lack of germ cell markers. The dead end (dnd) gene is a vertebrate-specific component of germplasm crucial for primordial germ cells (PGCs) migration and development in teleosts. In this study, we identified a dnd homologue (Smdnd) in turbot (Scophthalmus maximus) and investigated its expression pattern during embryogenesis and gonadal development. The deduced amino acid sequence of Smdnd shared several conserved motifs of Dnd homologues as well as high identity to other Dnd proteins. Phylogenetic analysis revealed that the SmDnd was closely related to its teleost counterparts. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization revealed that Smdnd transcripts could be exclusively detected in germ cells, including presumptive PGC and adult male and female germ cells. In addition, an interesting sexually dimorphic expression of Smdnd during gonadal development was observed by real-time PCR. Female turbot showed greater (P < 0.05) Smdnd expression than male before sex maturation. This difference reduced gradually due to the upregulation of Smdnd in the male during the period corresponding to spermatogonia proliferation and meiosis. These results indicate that Smdnd can be used as a germ cell marker in turbot. In addition, the temporal and sex differences in Smdnd expression indicate that this gene may play different roles in gonadal development in both sexes. (C) 2013 Elsevier Inc. All rights reserved.
关键词	Dead End Scophthalmus Maximus Teleost Germ Cell Gonadal Development
学科领域	Reproductive Biology ; Veterinary Sciences
DOI	10.1016/j.theriogenology.2013.06.016
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000323857200017
WOS关键词	MESSENGER-RNA ; ZEBRAFISH MIR-430 ; CELL DEVELOPMENT ; MIGRATION ; FISH ; VASA
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16702
专题	海洋生物技术研发中心实验海洋生物学重点实验室
通讯作者	Li, J
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 3.State Ocean Adm, Inst Oceanog 3, Xiamen, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lin, F.,Zhao, C. Y.,Xu, S. H.,et al. Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)[J]. THERIOGENOLOGY,2013,80(6):665-672.
APA	Lin, F..,Zhao, C. Y..,Xu, S. H..,Ma, D. Y..,Xiao, Z. Z..,...&Li, J.(2013).Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus).THERIOGENOLOGY,80(6),665-672.
MLA	Lin, F.,et al."Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)".THERIOGENOLOGY 80.6(2013):665-672.

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