Metabarcoding analysis of harmful algal species in Jiaozhou Bay

doi:10.1016/j.hal.2020.101772

	Metabarcoding analysis of harmful algal species in Jiaozhou Bay
	Liu, Shuya 1,2,4; Gibson, Kate 5; Cui, Zongmei 1,2,3,4; Chen, Yang 1,2,3,4; Sun, Xiaoxia2,4,6 ; Chen, Nansheng 1,2,4,5
	2020-02-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	92 页码:12
通讯作者	Chen, Nansheng(chenn@sfu.ca)
摘要	Accurate detection of the composition and dynamics of harmful algal bloom (HAB) species is critical for studying the mechanisms of HAB formation and for developing means for predicting the occurrences of HABs. Jiaozhou Bay is an epitome of China's coastal ecosystem and an ideal site for HAB research with the accumulation of decades of historical investigation records. Nevertheless, most of these earlier studies on phytoplankton communities applied primarily morphology-based approaches with limited resolution in phytoplankton species identification, especially for those with small-sized cells and for cryptic species. Through analyzing samples collected at 12 spatially isolated locations using metabarcoding methods, 89 phytoplankton species, including 34 Bacillariophyta, 25 Dinoflagellata, 7 Cryptophyta, 11 Chlorophyta, 8 Ochrophyta and 2 Haptophyta species were detected. Of those, 70 species had never been reported in Jiaozhou Bay in the previous expedition investigations, demonstrating the strength of the metabarcoding analysis approach. The distribution of many algal species demonstrated unique patterns, which were likely influenced by interactions among phytoplankton species or by predation by groups such as Ciliophora and Cercozoa, in addition to environmental factors such as temperature and nutritional conditions. Among these algal species, 28 were annotated as HAB species, among which 13 were reported for the very first time in Jiaozhou Bay including a mixtotrophic dianoflagellate Heterocapsa rotundata and a chain-forming diatom Skeletonema marinoi, both ranked among the top 10 most abundant ASVs. The present study represents a first attempt to study HAB species and other phytoplankton species in Jiaozhou Bay using the metabarcoding approach, which revealed substantially more algal species in Jiaozhou Bay than previously identified and sets a solid foundation for further research on the mechanisms of HAB formation.
关键词	Jiaozhou Bay Harmful algal bloom species Metabarcoding technique 18S rDNA Amplicon sequence variant (ASV)
DOI	10.1016/j.hal.2020.101772
收录类别	SCI
语种	英语
资助项目	Qingdao National Laboratory for Marine Science and Technology[QNLM201704] ; Natural Science Foundation of China[41906118] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000519335700013
出版者	ELSEVIER
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167006
专题	海洋生态与环境科学重点实验室胶州湾海洋生态系统国家野外研究站
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Liu, Shuya,Gibson, Kate,Cui, Zongmei,et al. Metabarcoding analysis of harmful algal species in Jiaozhou Bay[J]. HARMFUL ALGAE,2020,92:12.
APA	Liu, Shuya,Gibson, Kate,Cui, Zongmei,Chen, Yang,Sun, Xiaoxia,&Chen, Nansheng.(2020).Metabarcoding analysis of harmful algal species in Jiaozhou Bay.HARMFUL ALGAE,92,12.
MLA	Liu, Shuya,et al."Metabarcoding analysis of harmful algal species in Jiaozhou Bay".HARMFUL ALGAE 92(2020):12.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Metabarcoding analys（9477KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览