Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Metabarcoding analysis of harmful algal species in Jiaozhou Bay | |
Liu, Shuya1,2,4; Gibson, Kate5; Cui, Zongmei1,2,3,4; Chen, Yang1,2,3,4; Sun, Xiaoxia2,4,6 ; Chen, Nansheng1,2,4,5
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| 2020-02-01 | |
| Source Publication | HARMFUL ALGAE
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| ISSN | 1568-9883 |
| Volume | 92Pages:12 |
| Corresponding Author | Chen, Nansheng(chenn@sfu.ca) |
| Abstract | Accurate detection of the composition and dynamics of harmful algal bloom (HAB) species is critical for studying the mechanisms of HAB formation and for developing means for predicting the occurrences of HABs. Jiaozhou Bay is an epitome of China's coastal ecosystem and an ideal site for HAB research with the accumulation of decades of historical investigation records. Nevertheless, most of these earlier studies on phytoplankton communities applied primarily morphology-based approaches with limited resolution in phytoplankton species identification, especially for those with small-sized cells and for cryptic species. Through analyzing samples collected at 12 spatially isolated locations using metabarcoding methods, 89 phytoplankton species, including 34 Bacillariophyta, 25 Dinoflagellata, 7 Cryptophyta, 11 Chlorophyta, 8 Ochrophyta and 2 Haptophyta species were detected. Of those, 70 species had never been reported in Jiaozhou Bay in the previous expedition investigations, demonstrating the strength of the metabarcoding analysis approach. The distribution of many algal species demonstrated unique patterns, which were likely influenced by interactions among phytoplankton species or by predation by groups such as Ciliophora and Cercozoa, in addition to environmental factors such as temperature and nutritional conditions. Among these algal species, 28 were annotated as HAB species, among which 13 were reported for the very first time in Jiaozhou Bay including a mixtotrophic dianoflagellate Heterocapsa rotundata and a chain-forming diatom Skeletonema marinoi, both ranked among the top 10 most abundant ASVs. The present study represents a first attempt to study HAB species and other phytoplankton species in Jiaozhou Bay using the metabarcoding approach, which revealed substantially more algal species in Jiaozhou Bay than previously identified and sets a solid foundation for further research on the mechanisms of HAB formation. |
| Keyword | Jiaozhou Bay Harmful algal bloom species Metabarcoding technique 18S rDNA Amplicon sequence variant (ASV) |
| DOI | 10.1016/j.hal.2020.101772 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Qingdao National Laboratory for Marine Science and Technology[QNLM201704] ; Natural Science Foundation of China[41906118] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc] |
| WOS Research Area | Marine & Freshwater Biology |
| WOS Subject | Marine & Freshwater Biology |
| WOS ID | WOS:000519335700013 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167006 |
| Collection | 海洋生态与环境科学重点实验室 胶州湾海洋生态系统国家野外研究站 |
| Corresponding Author | Chen, Nansheng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China |
| First Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS |
| Corresponding Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS |
| Recommended Citation GB/T 7714 | Liu, Shuya,Gibson, Kate,Cui, Zongmei,et al. Metabarcoding analysis of harmful algal species in Jiaozhou Bay[J]. HARMFUL ALGAE,2020,92:12. |
| APA | Liu, Shuya,Gibson, Kate,Cui, Zongmei,Chen, Yang,Sun, Xiaoxia,&Chen, Nansheng.(2020).Metabarcoding analysis of harmful algal species in Jiaozhou Bay.HARMFUL ALGAE,92,12. |
| MLA | Liu, Shuya,et al."Metabarcoding analysis of harmful algal species in Jiaozhou Bay".HARMFUL ALGAE 92(2020):12. |
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| Metabarcoding analys（9477KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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