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Metabarcoding analysis of harmful algal species in Jiaozhou Bay
Liu, Shuya1,2,4; Gibson, Kate5; Cui, Zongmei1,2,3,4; Chen, Yang1,2,3,4; Sun, Xiaoxia2,4,6; Chen, Nansheng1,2,4,5
2020-02-01
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume92Pages:12
Corresponding AuthorChen, Nansheng(chenn@sfu.ca)
AbstractAccurate detection of the composition and dynamics of harmful algal bloom (HAB) species is critical for studying the mechanisms of HAB formation and for developing means for predicting the occurrences of HABs. Jiaozhou Bay is an epitome of China's coastal ecosystem and an ideal site for HAB research with the accumulation of decades of historical investigation records. Nevertheless, most of these earlier studies on phytoplankton communities applied primarily morphology-based approaches with limited resolution in phytoplankton species identification, especially for those with small-sized cells and for cryptic species. Through analyzing samples collected at 12 spatially isolated locations using metabarcoding methods, 89 phytoplankton species, including 34 Bacillariophyta, 25 Dinoflagellata, 7 Cryptophyta, 11 Chlorophyta, 8 Ochrophyta and 2 Haptophyta species were detected. Of those, 70 species had never been reported in Jiaozhou Bay in the previous expedition investigations, demonstrating the strength of the metabarcoding analysis approach. The distribution of many algal species demonstrated unique patterns, which were likely influenced by interactions among phytoplankton species or by predation by groups such as Ciliophora and Cercozoa, in addition to environmental factors such as temperature and nutritional conditions. Among these algal species, 28 were annotated as HAB species, among which 13 were reported for the very first time in Jiaozhou Bay including a mixtotrophic dianoflagellate Heterocapsa rotundata and a chain-forming diatom Skeletonema marinoi, both ranked among the top 10 most abundant ASVs. The present study represents a first attempt to study HAB species and other phytoplankton species in Jiaozhou Bay using the metabarcoding approach, which revealed substantially more algal species in Jiaozhou Bay than previously identified and sets a solid foundation for further research on the mechanisms of HAB formation.
KeywordJiaozhou Bay Harmful algal bloom species Metabarcoding technique 18S rDNA Amplicon sequence variant (ASV)
DOI10.1016/j.hal.2020.101772
Indexed BySCI
Language英语
Funding ProjectQingdao National Laboratory for Marine Science and Technology[QNLM201704] ; Natural Science Foundation of China[41906118] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc]
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000519335700013
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167006
Collection海洋生态与环境科学重点实验室
胶州湾海洋生态系统国家野外研究站
Corresponding AuthorChen, Nansheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Liu, Shuya,Gibson, Kate,Cui, Zongmei,et al. Metabarcoding analysis of harmful algal species in Jiaozhou Bay[J]. HARMFUL ALGAE,2020,92:12.
APA Liu, Shuya,Gibson, Kate,Cui, Zongmei,Chen, Yang,Sun, Xiaoxia,&Chen, Nansheng.(2020).Metabarcoding analysis of harmful algal species in Jiaozhou Bay.HARMFUL ALGAE,92,12.
MLA Liu, Shuya,et al."Metabarcoding analysis of harmful algal species in Jiaozhou Bay".HARMFUL ALGAE 92(2020):12.
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