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Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay
Zhou, Shuhong1,3; Wang, Mengqiang1; Liu, Mei1; Jiang, Keyong1; Wang, Baojie1; Wang, Lei1,2,4
2020-05-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume521Pages:6
Corresponding AuthorWang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
AbstractEnterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about them early. In this study, we describe a novel, sensitive and specific rapid isothermal recombinase polymerase amplification assay (RPA) for detecting EHP. We evaluated the ability, including the specificity and sensitivity, of the EHP-RPA assay to detect EHP. By optimizing the reaction time and temperature, and screening primers based on the conserved small subunit ribosomal RNA gene, we determined the optimal reaction conditions. The RPA could be performed at 30 degrees C for 40 min without cross-reactivity with other pathogens. Sensitivity assay indicated that the limit of detection for EHP-RPA, PCR and real-time PCR was 8 x 10(2), 8 x 10(2) and 8 x 10(1) copies/mu L, respectively. Using 18 samples collected from shrimp farms, we demonstrated that RPA enables simple, convenient and rapid EHP detection, especially when laboratory equipment is lacking. This new RPA method is easy to implement and has great potential for use in both laboratory and field testing applications.
KeywordPenaeus vannamei Enterocytozoon hepatopenaei Recombinase polymerase amplification
DOI10.1016/j.aquaculture.2020.734987
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2019YFD0900401] ; National Natural Science Foundation of China-Joint Fund of Shandong People's Government[2017T3011]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000521282400061
PublisherELSEVIER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/166997
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Baojie; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,et al. Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay[J]. AQUACULTURE,2020,521:6.
APA Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,Jiang, Keyong,Wang, Baojie,&Wang, Lei.(2020).Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay.AQUACULTURE,521,6.
MLA Zhou, Shuhong,et al."Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay".AQUACULTURE 521(2020):6.
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