Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay | |
Zhou, Shuhong1,3; Wang, Mengqiang1; Liu, Mei1; Jiang, Keyong1; Wang, Baojie1; Wang, Lei1,2,4 | |
2020-05-15 | |
发表期刊 | AQUACULTURE |
ISSN | 0044-8486 |
卷号 | 521页码:6 |
通讯作者 | Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn) |
摘要 | Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about them early. In this study, we describe a novel, sensitive and specific rapid isothermal recombinase polymerase amplification assay (RPA) for detecting EHP. We evaluated the ability, including the specificity and sensitivity, of the EHP-RPA assay to detect EHP. By optimizing the reaction time and temperature, and screening primers based on the conserved small subunit ribosomal RNA gene, we determined the optimal reaction conditions. The RPA could be performed at 30 degrees C for 40 min without cross-reactivity with other pathogens. Sensitivity assay indicated that the limit of detection for EHP-RPA, PCR and real-time PCR was 8 x 10(2), 8 x 10(2) and 8 x 10(1) copies/mu L, respectively. Using 18 samples collected from shrimp farms, we demonstrated that RPA enables simple, convenient and rapid EHP detection, especially when laboratory equipment is lacking. This new RPA method is easy to implement and has great potential for use in both laboratory and field testing applications. |
关键词 | Penaeus vannamei Enterocytozoon hepatopenaei Recombinase polymerase amplification |
DOI | 10.1016/j.aquaculture.2020.734987 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key R&D Program of China[2019YFD0900401] ; National Natural Science Foundation of China-Joint Fund of Shandong People's Government[2017T3011] |
WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
WOS类目 | Fisheries ; Marine & Freshwater Biology |
WOS记录号 | WOS:000521282400061 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/166997 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Wang, Baojie; Wang, Lei |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
推荐引用方式 GB/T 7714 | Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,et al. Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay[J]. AQUACULTURE,2020,521:6. |
APA | Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,Jiang, Keyong,Wang, Baojie,&Wang, Lei.(2020).Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay.AQUACULTURE,521,6. |
MLA | Zhou, Shuhong,et al."Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay".AQUACULTURE 521(2020):6. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Rapid detection of E(898KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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