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Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis
Gao, Yang1,2; Zhang, Huan1; Gao, Qiang1; Wang, Lingling1; Zhang, Fuchong3; Siva, Vinu S.1,2; Zhou, Zhi1; Song, Linsheng1; Zhang, Shicui4; Zhang, FC
2013-03-08
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:3Pages:e58453
SubtypeArticle
AbstractJiyan-1 puffer, the F1 hybrid of Takifugu rubripes and Takifugu flavidus, displays obvious heterosis in the growth performance, flavor and stress tolerance. In the present study, comparative analysis for the transcriptomes of T. rubripes, T. flavidus and Jiyan-1 was performed aiming to reveal the possible mechanisms of heterosis in pufferfish. Whole transcriptomes were sequenced using the SOLiD4 platform, and a total of 44,305 transcripts corresponding to 18,164 genes were identified collectively. A total of 14,148 transcripts were differentially expressed. By comparing the gene expression patterns of the three samples, the coexistence of overdominance, dominance, underdominance and additivity was observed in the gene action modes of Jiyan-1. There were 2,237 transcripts in the intersection of the differentially expressed transcripts from Jiyan-1 versus T. rubripes and Jiyan-1 versus T. flavidus, among which 213 transcripts were also in the T. rubripes versus T. flavidus. The potential functions of the remaining 2,024 transcripts were mainly associated with metabolic process, nucleotide binding and catalytic activity. The enrichment results indicated metabolism was the most activated biological function in the heterosis. In addition, 35 KEGG pathways were retrieved as affiliated with more than three differentially expressed transcripts and 8,579 potentially novel transcript isoforms were identified for Jiyan-1. The present study revealed the coexistence of multiple gene actions in the hybrid puffer, indicated the importance of metabolism, ion binding function and kinase activity, as well as provided a list of candidate genes and pathways for heterosis. It could be helpful for the better understanding of the determination and regulation mechanisms of heterosis.; Jiyan-1 puffer, the F1 hybrid of Takifugu rubripes and Takifugu flavidus, displays obvious heterosis in the growth performance, flavor and stress tolerance. In the present study, comparative analysis for the transcriptomes of T. rubripes, T. flavidus and Jiyan-1 was performed aiming to reveal the possible mechanisms of heterosis in pufferfish. Whole transcriptomes were sequenced using the SOLiD4 platform, and a total of 44,305 transcripts corresponding to 18,164 genes were identified collectively. A total of 14,148 transcripts were differentially expressed. By comparing the gene expression patterns of the three samples, the coexistence of overdominance, dominance, underdominance and additivity was observed in the gene action modes of Jiyan-1. There were 2,237 transcripts in the intersection of the differentially expressed transcripts from Jiyan-1 versus T. rubripes and Jiyan-1 versus T. flavidus, among which 213 transcripts were also in the T. rubripes versus T. flavidus. The potential functions of the remaining 2,024 transcripts were mainly associated with metabolic process, nucleotide binding and catalytic activity. The enrichment results indicated metabolism was the most activated biological function in the heterosis. In addition, 35 KEGG pathways were retrieved as affiliated with more than three differentially expressed transcripts and 8,579 potentially novel transcript isoforms were identified for Jiyan-1. The present study revealed the coexistence of multiple gene actions in the hybrid puffer, indicated the importance of metabolism, ion binding function and kinase activity, as well as provided a list of candidate genes and pathways for heterosis. It could be helpful for the better understanding of the determination and regulation mechanisms of heterosis.
Subject AreaScience & Technology - Other Topics
DOI10.1371/journal.pone.0058453
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000318679900070
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/16665
Collection实验海洋生物学重点实验室
海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorZhang, FC
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Ocean & Fishery Sci Res Inst Hebei Prov, Qinhuangdao, Peoples R China
4.Ocean Univ China, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gao, Yang,Zhang, Huan,Gao, Qiang,et al. Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis[J]. PLOS ONE,2013,8(3):e58453.
APA Gao, Yang.,Zhang, Huan.,Gao, Qiang.,Wang, Lingling.,Zhang, Fuchong.,...&Zhang, FC.(2013).Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis.PLOS ONE,8(3),e58453.
MLA Gao, Yang,et al."Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis".PLOS ONE 8.3(2013):e58453.
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