The suppressor of cytokine signaling 2 (SOCS2) modulating the neurotransmitters release in Eriocheir sinensis

doi:10.1016/j.fsi.2013.04.007

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	The suppressor of cytokine signaling 2 (SOCS2) modulating the neurotransmitters release in Eriocheir sinensis
	Zhang, Ying 1,2; Zhou, Zhi 1; Wang, Lingling 1; Liu, Rui 1; Song, Linsheng 1; Song, LS
	2013-07-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	35 期号:1 页码:101-106
文章类型	Article
摘要	The SOCS proteins appear to define an important mechanism for the negative regulation of the cytokine -JAK-STAT pathway. In the present study, the mRNA expression profiles of a SOCS2 from Chinese mitten crab Eriocheir sinensis (EsSOCS2) after pentachlorophenol (PCP) treatment or RNA interference (RNAi) were analyzed to understand its possible regulatory roles in modulating the neurotransmitter release. The EsSOCS2 expression level in the PCP treated group was significantly higher than that of blank at 1.5, 3, 12 and 24 h after exposure, suggesting that EsSOCS2 might be involved in controlling and reducing neuronal cell damage resulted from PCP treatment. After the expression of EsSOCS2 gene was silenced by RNAi the concentrations of catecholamines and nitric oxide (NO) were examined to evaluate the modulation of EsSOCS2 on the release of neurotransmitters. At 48 h after the treatment with sequence-specific dsRNA targeting E5SOCS2, the expression of E5SOCS2 was reduced to half compared to the original level, and the concentrations of norepinephrine and NO increased, while dopamine decreased significantly in haemolymph. The preliminary results indicated that EsSOCS2 regulated catecholaminergic neuroendocrine system to release catecholamines into haemolymph and might be an important feedback inhibitor of tyrosine kinase signaling pathways in crab, which subsequently regulated NO synthesis and prevented excessive NO release. This information is helpful to further understand the modulation of E5SOCS2 on neurotransmitter release in crab. (C) 2013 Elsevier Ltd. All rights reserved.; The SOCS proteins appear to define an important mechanism for the negative regulation of the cytokine -JAK-STAT pathway. In the present study, the mRNA expression profiles of a SOCS2 from Chinese mitten crab Eriocheir sinensis (EsSOCS2) after pentachlorophenol (PCP) treatment or RNA interference (RNAi) were analyzed to understand its possible regulatory roles in modulating the neurotransmitter release. The EsSOCS2 expression level in the PCP treated group was significantly higher than that of blank at 1.5, 3, 12 and 24 h after exposure, suggesting that EsSOCS2 might be involved in controlling and reducing neuronal cell damage resulted from PCP treatment. After the expression of EsSOCS2 gene was silenced by RNAi the concentrations of catecholamines and nitric oxide (NO) were examined to evaluate the modulation of EsSOCS2 on the release of neurotransmitters. At 48 h after the treatment with sequence-specific dsRNA targeting E5SOCS2, the expression of E5SOCS2 was reduced to half compared to the original level, and the concentrations of norepinephrine and NO increased, while dopamine decreased significantly in haemolymph. The preliminary results indicated that EsSOCS2 regulated catecholaminergic neuroendocrine system to release catecholamines into haemolymph and might be an important feedback inhibitor of tyrosine kinase signaling pathways in crab, which subsequently regulated NO synthesis and prevented excessive NO release. This information is helpful to further understand the modulation of E5SOCS2 on neurotransmitter release in crab. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Eriocheir Sinensis Suppressors Of Cytokine Signaling 2 Pentachlorophenol Catecholamine Nitric Oxide
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2013.04.007
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000320749700013
WOS关键词	NITRIC-OXIDE ; IMMUNE-SYSTEM ; INNATE IMMUNITY ; PENTACHLOROPHENOL ; BRAIN ; NEUROENDOCRINE ; MECHANISMS ; STRESS ; FAMILY ; CELLS
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16660
专题	实验海洋生物学重点实验室
通讯作者	Song, LS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Shandong Marine Fisheries Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Shandong, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Ying,Zhou, Zhi,Wang, Lingling,et al. The suppressor of cytokine signaling 2 (SOCS2) modulating the neurotransmitters release in Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(1):101-106.
APA	Zhang, Ying,Zhou, Zhi,Wang, Lingling,Liu, Rui,Song, Linsheng,&Song, LS.(2013).The suppressor of cytokine signaling 2 (SOCS2) modulating the neurotransmitters release in Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,35(1),101-106.
MLA	Zhang, Ying,et al."The suppressor of cytokine signaling 2 (SOCS2) modulating the neurotransmitters release in Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 35.1(2013):101-106.

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