Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix | |
Wang, Chao1; Yue, Xin1; Lu, Xia1,2; Liu, Baozhong1 ; Liu, BZ
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| 2013 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 34期号:1页码:91-99 |
| 文章类型 | Article |
| 摘要 | Catalase (CAT) can effectively eliminate H2O2 and maintain the redox balance of immune system, which is essential for innate immunity. A catalase gene was cloned and its potential role in immune system was investigated in the clam, Meretrix meretrix. The catalase (MmeCAT) gene had an open reading frame of 1533 bp encoding 511 amino acids which showed high identity with that of molluscs. The distribution of MmeCAT in clam tissues was examined and the mRNA, protein expression and CAT activity paralleled with each other, with the highest expression in hepatopancreas. In response to H2O2 challenge, MmeCAT mRNA showed significantly higher expression at 12 h and 24 h post-challenge in experimental clams than in control clams (P < 0.05). Meanwhile, the protein expression in experimental clams was increased to about 3 times as much as that in control clams at 6 h post-challenge. After injection with a Vibrio parahaemolyticus-related bacterium (MM21), the expression of MmeCAT mRNA was significantly up-regulated at 12 h and 24 h post-injection (P < 0.05). It suggested that MmeCAT might be involved in the immune response to Vibrio infection. To better understand the role of MmeCAT in immune system, its mRNA expression was compared between a Vibrio-resistant population and a control population after immersion challenge with MM21. The continuously increased transcription in resistant population suggested MmeCAT could benefit the immune system of clams to defend against pathogen infection. Our study indicated that the redox balance was essential for M. meretrix to resist pathogen infection. (c) 2012 Elsevier Ltd. All rights reserved.; Catalase (CAT) can effectively eliminate H2O2 and maintain the redox balance of immune system, which is essential for innate immunity. A catalase gene was cloned and its potential role in immune system was investigated in the clam, Meretrix meretrix. The catalase (MmeCAT) gene had an open reading frame of 1533 bp encoding 511 amino acids which showed high identity with that of molluscs. The distribution of MmeCAT in clam tissues was examined and the mRNA, protein expression and CAT activity paralleled with each other, with the highest expression in hepatopancreas. In response to H2O2 challenge, MmeCAT mRNA showed significantly higher expression at 12 h and 24 h post-challenge in experimental clams than in control clams (P < 0.05). Meanwhile, the protein expression in experimental clams was increased to about 3 times as much as that in control clams at 6 h post-challenge. After injection with a Vibrio parahaemolyticus-related bacterium (MM21), the expression of MmeCAT mRNA was significantly up-regulated at 12 h and 24 h post-injection (P < 0.05). It suggested that MmeCAT might be involved in the immune response to Vibrio infection. To better understand the role of MmeCAT in immune system, its mRNA expression was compared between a Vibrio-resistant population and a control population after immersion challenge with MM21. The continuously increased transcription in resistant population suggested MmeCAT could benefit the immune system of clams to defend against pathogen infection. Our study indicated that the redox balance was essential for M. meretrix to resist pathogen infection. (c) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Meretrix Meretrix Cat Redox Balance Oxidative Stress Vibrio Parahaemolyticus |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2012.10.013 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000314743600011 |
| WOS关键词 | NF-KAPPA-B ; GLUTATHIONE-PEROXIDASE ; MOLECULAR CHARACTERIZATION ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; HOST PROTECTION ; CELL-DEATH ; EXPRESSION ; GENE ; CLONING |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16659 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Yue, Xin,Lu, Xia,et al. The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):91-99. |
| APA | Wang, Chao,Yue, Xin,Lu, Xia,Liu, Baozhong,&Liu, BZ.(2013).The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,34(1),91-99. |
| MLA | Wang, Chao,et al."The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):91-99. |
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