Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation | |
Zhou, Zhi1; Wang, Mengqiang1 ; Zhao, Jianmin1; Wang, Lingling1; Gao, Yang1,2; Zhang, Huan1; Liu, Rui1; Song, Linsheng1; Wang, LL
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| 2013-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 34期号:5页码:1209-1215 |
| 文章类型 | Article |
| 摘要 | The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved.; The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Rel Nf-kappa b Transcription Activity Immunomodulation Scallop |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.01.009 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000317870600023 |
| WOS关键词 | C-TYPE LECTIN ; DROSOPHILA-MELANOGASTER ; SIGNALING PATHWAYS ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; KINASE COMPLEX ; ACTIVATION ; HEMOCYTES ; PROTEIN ; EXPRESSION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16649 |
| 专题 | 实验海洋生物学重点实验室 海洋环境腐蚀与生物污损重点实验室 |
| 通讯作者 | Wang, LL |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhou, Zhi,Wang, Mengqiang,Zhao, Jianmin,et al. The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1209-1215. |
| APA | Zhou, Zhi.,Wang, Mengqiang.,Zhao, Jianmin.,Wang, Lingling.,Gao, Yang.,...&Wang, LL.(2013).The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation.FISH & SHELLFISH IMMUNOLOGY,34(5),1209-1215. |
| MLA | Zhou, Zhi,et al."The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1209-1215. |
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