Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta) | |
Shan, Ti Feng; Pang, Shao Jun; Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China | |
2010-07-01 | |
发表期刊 | PHYCOLOGICAL RESEARCH |
ISSN | 1322-0829 |
卷号 | 58期号:3页码:171-176 |
文章类型 | Article |
摘要 | P>In our microsatellite analysis of three male and three female gametophytes of Undaria pinnatifida (Harv.) Suringar, a microsatellite marker (part of the locus Up-AC-2A8, GenBank accession no. AY738602.1) was only polymerase chain reaction-amplified in three female gametophytes. This putative female-specific marker was further tested by the use of 32 male and 21 female gametophytes maintained in the Marine Biological Culture Collection Centre, China. In addition, three sporophytes were included for confirmation. Results showed that the marker was present in all of the female gametophytes and sporophyte cultures, but absent in all of the male gametophytes. To our knowledge, this is the first sex-related marker ever reported in U. pinnatifida. The discovery of this marker will accelerate gender identification and shed light on our understanding of the mechanisms of sex determination at a molecular level in this commercially important seaweed.; P>In our microsatellite analysis of three male and three female gametophytes of Undaria pinnatifida (Harv.) Suringar, a microsatellite marker (part of the locus Up-AC-2A8, GenBank accession no. AY738602.1) was only polymerase chain reaction-amplified in three female gametophytes. This putative female-specific marker was further tested by the use of 32 male and 21 female gametophytes maintained in the Marine Biological Culture Collection Centre, China. In addition, three sporophytes were included for confirmation. Results showed that the marker was present in all of the female gametophytes and sporophyte cultures, but absent in all of the male gametophytes. To our knowledge, this is the first sex-related marker ever reported in U. pinnatifida. The discovery of this marker will accelerate gender identification and shed light on our understanding of the mechanisms of sex determination at a molecular level in this commercially important seaweed. |
关键词 | Gametophyte Microsatellite Marker Sex Determination Sex-related Undaria Pinnatifida |
学科领域 | Marine & Freshwater Biology |
DOI | 10.1111/j.1440-1835.2010.00575.x |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000279171500002 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/1663 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China |
作者单位 | Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Shan, Ti Feng,Pang, Shao Jun,Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China. Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta)[J]. PHYCOLOGICAL RESEARCH,2010,58(3):171-176. |
APA | Shan, Ti Feng,Pang, Shao Jun,&Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China.(2010).Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta).PHYCOLOGICAL RESEARCH,58(3),171-176. |
MLA | Shan, Ti Feng,et al."Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta)".PHYCOLOGICAL RESEARCH 58.3(2010):171-176. |
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