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Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta)
Shan, Ti Feng; Pang, Shao Jun; Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China
2010-07-01
发表期刊PHYCOLOGICAL RESEARCH
ISSN1322-0829
卷号58期号:3页码:171-176
文章类型Article
摘要P>In our microsatellite analysis of three male and three female gametophytes of Undaria pinnatifida (Harv.) Suringar, a microsatellite marker (part of the locus Up-AC-2A8, GenBank accession no. AY738602.1) was only polymerase chain reaction-amplified in three female gametophytes. This putative female-specific marker was further tested by the use of 32 male and 21 female gametophytes maintained in the Marine Biological Culture Collection Centre, China. In addition, three sporophytes were included for confirmation. Results showed that the marker was present in all of the female gametophytes and sporophyte cultures, but absent in all of the male gametophytes. To our knowledge, this is the first sex-related marker ever reported in U. pinnatifida. The discovery of this marker will accelerate gender identification and shed light on our understanding of the mechanisms of sex determination at a molecular level in this commercially important seaweed.; P>In our microsatellite analysis of three male and three female gametophytes of Undaria pinnatifida (Harv.) Suringar, a microsatellite marker (part of the locus Up-AC-2A8, GenBank accession no. AY738602.1) was only polymerase chain reaction-amplified in three female gametophytes. This putative female-specific marker was further tested by the use of 32 male and 21 female gametophytes maintained in the Marine Biological Culture Collection Centre, China. In addition, three sporophytes were included for confirmation. Results showed that the marker was present in all of the female gametophytes and sporophyte cultures, but absent in all of the male gametophytes. To our knowledge, this is the first sex-related marker ever reported in U. pinnatifida. The discovery of this marker will accelerate gender identification and shed light on our understanding of the mechanisms of sex determination at a molecular level in this commercially important seaweed.
关键词Gametophyte Microsatellite Marker Sex Determination Sex-related Undaria Pinnatifida
学科领域Marine & Freshwater Biology
DOI10.1111/j.1440-1835.2010.00575.x
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收录类别SCI
语种英语
WOS记录号WOS:000279171500002
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/1663
专题实验海洋生物学重点实验室
通讯作者Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China
作者单位Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所
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GB/T 7714
Shan, Ti Feng,Pang, Shao Jun,Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China. Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta)[J]. PHYCOLOGICAL RESEARCH,2010,58(3):171-176.
APA Shan, Ti Feng,Pang, Shao Jun,&Pang, SJ, Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China.(2010).Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta).PHYCOLOGICAL RESEARCH,58(3),171-176.
MLA Shan, Ti Feng,et al."Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta)".PHYCOLOGICAL RESEARCH 58.3(2010):171-176.
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