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Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications
Lin, A-Peng1; Wang, Guang-Ce1; Zhou, Wen-Qin2; Wang, GC
2013-08-01
发表期刊PHYSIOLOGIA PLANTARUM
ISSN0031-9317
卷号148期号:4页码:582-589
文章类型Article
摘要Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.; Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.
学科领域Plant Sciences
DOI10.1111/ppl.12008
URL查看原文
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000321697300012
WOS关键词ION-SELECTIVE MICROELECTRODES ; INORGANIC CARBON UTILIZATION ; CHARA-CORALLINA ; PHOTOSYNTHETIC UTILIZATION ; PLASMA-MEMBRANE ; PROTON FLUXES ; SINGLE-CELL ; BICARBONATE ; SEAGRASSES ; TRANSPORT
WOS标题词Science & Technology ; Life Sciences & Biomedicine
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16633
专题实验海洋生物学重点实验室
通讯作者Wang, GC
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Lin, A-Peng,Wang, Guang-Ce,Zhou, Wen-Qin,et al. Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications[J]. PHYSIOLOGIA PLANTARUM,2013,148(4):582-589.
APA Lin, A-Peng,Wang, Guang-Ce,Zhou, Wen-Qin,&Wang, GC.(2013).Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications.PHYSIOLOGIA PLANTARUM,148(4),582-589.
MLA Lin, A-Peng,et al."Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications".PHYSIOLOGIA PLANTARUM 148.4(2013):582-589.
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