Shrimp MyD88 responsive to bacteria and white spot syndrome virus

doi:10.1016/j.fsi.2012.11.034

IOCAS-IR > 实验海洋生物学重点实验室

	Shrimp MyD88 responsive to bacteria and white spot syndrome virus
	Wen, Rong 1,2; Li, Fuhua 1; Sun, Zheng 3; Li, Shihao 1; Xiang, Jianhai1 ; Li, FH
	2013-02-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	34 期号:2 页码:574-581
文章类型	Article
摘要	The myeloid differentiation factor 88 (MyD88) is an important adapter protein which links members of the toll-like receptor (TLR) to the downstream components to activate related signaling pathways. In the present study, a MyD88 homolog (FcMyD88) was cloned from penaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (DD) and a typical TLR and interleukin-1 receptor (IL-1R)-related (TIR) domain. Homology analysis revealed that the predicted amino acid (aa) sequence of FcMyD88 shared high similarities with a variety of previously reported MyD88s. The time-dependent expression patterns of FcMyD88 in cephalothoraxes of shrimp injected with Vibrio anguillarum (Gram-negative bacteria, G(-)), Micrococcus lysodeikticu (Gram-positive bacteria, G(+)) and white syndrome spot virus (WSSV) were analyzed at transcription and protein level by real-time PCR and western blotting, respectively. The expression level of FcMyD88 mRNA was significantly up-regulated at one hour (h), 12 h and 24 h after stimulation with both V anguillarum and M. lysodeikticu. The expression level of FcMyD88 protein was 2-fold up-regulated at 12 h post injection (hpi) of inactivated V. anguillarum while it didn't change after M. lysodeikticu injection during this period. After WSSV injection, the expression level of FcMyD88 mRNA remained relatively constant, while the FcMyD88 protein was significantly up-regulated at 12 and 24 hpi. These results suggested that the MyD88-dependent signaling pathway could be involved in the defense of both bacteria and WSSV infection. (C) 2012 Elsevier Ltd. All rights reserved.; The myeloid differentiation factor 88 (MyD88) is an important adapter protein which links members of the toll-like receptor (TLR) to the downstream components to activate related signaling pathways. In the present study, a MyD88 homolog (FcMyD88) was cloned from penaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (DD) and a typical TLR and interleukin-1 receptor (IL-1R)-related (TIR) domain. Homology analysis revealed that the predicted amino acid (aa) sequence of FcMyD88 shared high similarities with a variety of previously reported MyD88s. The time-dependent expression patterns of FcMyD88 in cephalothoraxes of shrimp injected with Vibrio anguillarum (Gram-negative bacteria, G(-)), Micrococcus lysodeikticu (Gram-positive bacteria, G(+)) and white syndrome spot virus (WSSV) were analyzed at transcription and protein level by real-time PCR and western blotting, respectively. The expression level of FcMyD88 mRNA was significantly up-regulated at one hour (h), 12 h and 24 h after stimulation with both V anguillarum and M. lysodeikticu. The expression level of FcMyD88 protein was 2-fold up-regulated at 12 h post injection (hpi) of inactivated V. anguillarum while it didn't change after M. lysodeikticu injection during this period. After WSSV injection, the expression level of FcMyD88 mRNA remained relatively constant, while the FcMyD88 protein was significantly up-regulated at 12 and 24 hpi. These results suggested that the MyD88-dependent signaling pathway could be involved in the defense of both bacteria and WSSV infection. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Myd88 Expression Immunity Penaeid Shrimp
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.11.034
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000315076100019
WOS关键词	TOLL RECEPTOR ; FENNEROPENAEUS-CHINENSIS ; SIGNALING PATHWAY ; CELL-DEVELOPMENT ; DENDRITIC CELLS ; WSSV INFECTION ; TLR ; EXPRESSION ; DROSOPHILA ; FAMILY
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16631
专题	实验海洋生物学重点实验室
通讯作者	Li, FH
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wen, Rong,Li, Fuhua,Sun, Zheng,et al. Shrimp MyD88 responsive to bacteria and white spot syndrome virus[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):574-581.
APA	Wen, Rong,Li, Fuhua,Sun, Zheng,Li, Shihao,Xiang, Jianhai,&Li, FH.(2013).Shrimp MyD88 responsive to bacteria and white spot syndrome virus.FISH & SHELLFISH IMMUNOLOGY,34(2),574-581.
MLA	Wen, Rong,et al."Shrimp MyD88 responsive to bacteria and white spot syndrome virus".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):574-581.

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