Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi

doi:10.1016/j.fsi.2013.04.033

IOCAS-IR > 实验海洋生物学重点实验室

	Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi
	Huang, Hai-Hong 1; Liu, Xiao-Lin 1; Xiang, Jian-Hai2 ; Wang, Ping 3; Liu, XL
	2013-08-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	35 期号:2 页码:328-333
文章类型	Article
摘要	To obtain Vibrio harveyi-resistant Litopenaeus vannamei shrimp used for study on immune response of shrimp avoid vibriosis, a three-round challenge selection procedure was applied. In this procedure, resistant shrimp were selected gradually via three rounds challenge experiment with a pathogenic strain of V harveyi at a median and controllable lethal dose of 96-h LD50 (the median lethal dose). After this procedure, the cumulative mortality of selected shrimp during 96 h after injection of V. harveyi at 2.0 x 10(6) cfu shrimp(-1) significantly decreased from 93.3% to 26.7%, the hours of beginning of death and the hours of attaining of the maximum cumulative mortality of shrimp prolonged from 4 h and 10 h to 8 h and 24 h, respectively. The LD50 of 6 h, 12 h, 24 h, 48 h and 96 h of selected shrimp significantly increased to 1.4 +/- 0.1 x 10(7) (p < 0.01), 5.5 +/- 0.4 x 10(6) (p < 0.01), 3.1 +/- 0.2 x 10(6) (p < 0.01), 2.7 +/- 0.1 x 10(6) (p < 0.01) and 2.7 +/- 0.1 x 10(6) cfu shrimp(-1) (p < 0.01), about 15.9, 15.3, 9.4, 10.0 and 10.4 times of that of normal shrimp, respectively. In conclusion, the resistance of shrimp to Vibrio significantly increased after the three-round challenge selection procedure. (C) 2013 Elsevier Ltd. All rights reserved.; To obtain Vibrio harveyi-resistant Litopenaeus vannamei shrimp used for study on immune response of shrimp avoid vibriosis, a three-round challenge selection procedure was applied. In this procedure, resistant shrimp were selected gradually via three rounds challenge experiment with a pathogenic strain of V harveyi at a median and controllable lethal dose of 96-h LD50 (the median lethal dose). After this procedure, the cumulative mortality of selected shrimp during 96 h after injection of V. harveyi at 2.0 x 10(6) cfu shrimp(-1) significantly decreased from 93.3% to 26.7%, the hours of beginning of death and the hours of attaining of the maximum cumulative mortality of shrimp prolonged from 4 h and 10 h to 8 h and 24 h, respectively. The LD50 of 6 h, 12 h, 24 h, 48 h and 96 h of selected shrimp significantly increased to 1.4 +/- 0.1 x 10(7) (p < 0.01), 5.5 +/- 0.4 x 10(6) (p < 0.01), 3.1 +/- 0.2 x 10(6) (p < 0.01), 2.7 +/- 0.1 x 10(6) (p < 0.01) and 2.7 +/- 0.1 x 10(6) cfu shrimp(-1) (p < 0.01), about 15.9, 15.3, 9.4, 10.0 and 10.4 times of that of normal shrimp, respectively. In conclusion, the resistance of shrimp to Vibrio significantly increased after the three-round challenge selection procedure. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Vibrio Harveyi Litopenaeus Vannamei Challenge Resistance Selection
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2013.04.033
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000322207500015
WOS关键词	WHITE SPOT SYNDROME ; IMMUNE-RESPONSE ; GENE-EXPRESSION ; SYNDROME VIRUS ; SHRIMP ; SUSCEPTIBILITY ; ALGINOLYTICUS ; INFECTION
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16628
专题	实验海洋生物学重点实验室
通讯作者	Liu, XL
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 3.Nanjiang Marine Biotechnol Co Ltd, Sanya 572000, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Huang, Hai-Hong,Liu, Xiao-Lin,Xiang, Jian-Hai,et al. Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):328-333.
APA	Huang, Hai-Hong,Liu, Xiao-Lin,Xiang, Jian-Hai,Wang, Ping,&Liu, XL.(2013).Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi.FISH & SHELLFISH IMMUNOLOGY,35(2),328-333.
MLA	Huang, Hai-Hong,et al."Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):328-333.

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