Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5 | |
| Cai, Jinling1; Wang, Guangce1,2; Cai, JL | |
| 2013-08-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| ISSN | 0960-8524 |
| 卷号 | 142页码:18-25 |
| 文章类型 | Article |
| 摘要 | In this study, transposon mutagenesis technology was utilized to enhance the hydrogen production capability of a wild marine photosynthetic bacterium Rhodovulum sulfidophilum P5. A mutant strain TH-253 that exhibited high hydrogen yield and weaker light absorption ability was screened. Under strong light conditions, the mutant produced more hydrogen than that of the WT. Under optimum light intensity (120 mu mol photons/m(2) s), the mutant achieved its highest hydrogen yield (1436 +/- 44 mL H-2/L, about 3.21 +/- 0.10 mol H-2/mol acetate), which was 40.37% higher that of the WT. In continuous operation mode, the hydrogen yield (3.59 +/- 0.11 mol H-2/mol acetate) and average hydrogen production rate (16.91 +/- 0.46 mL H-2/L h) of the mutant were 43.40% and 45.07% higher than those of the WT, respectively. The mutant strain TH-253 may be used as an appropriate starting strain for future photosynthesis-based large scale hydrogen production. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.; In this study, transposon mutagenesis technology was utilized to enhance the hydrogen production capability of a wild marine photosynthetic bacterium Rhodovulum sulfidophilum P5. A mutant strain TH-253 that exhibited high hydrogen yield and weaker light absorption ability was screened. Under strong light conditions, the mutant produced more hydrogen than that of the WT. Under optimum light intensity (120 mu mol photons/m(2) s), the mutant achieved its highest hydrogen yield (1436 +/- 44 mL H-2/L, about 3.21 +/- 0.10 mol H-2/mol acetate), which was 40.37% higher that of the WT. In continuous operation mode, the hydrogen yield (3.59 +/- 0.11 mol H-2/mol acetate) and average hydrogen production rate (16.91 +/- 0.46 mL H-2/L h) of the mutant were 43.40% and 45.07% higher than those of the WT, respectively. The mutant strain TH-253 may be used as an appropriate starting strain for future photosynthesis-based large scale hydrogen production. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved. |
| 关键词 | Biological Hydrogen Production Transposon Rhodovulum Sulfidophilum Reduced Light Absorption |
| 学科领域 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| DOI | 10.1016/j.biortech.2013.05.009 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000322292800003 |
| WOS关键词 | RHODOPSEUDOMONAS-FAECALIS RLD-53 ; RHODOBACTER-SPHAEROIDES OU001 ; PHOTOSYNTHETIC BACTERIUM ; DEFICIENT MUTANT ; BIOHYDROGEN PRODUCTION ; RHODOSPIRILLUM-RUBRUM ; REDUCED PIGMENT ; CAPSULATUS ; PHOTO ; ACETATE |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16624 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Cai, JL |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Jinling,Wang, Guangce,Cai, JL. Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5[J]. BIORESOURCE TECHNOLOGY,2013,142:18-25. |
| APA | Cai, Jinling,Wang, Guangce,&Cai, JL.(2013).Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5.BIORESOURCE TECHNOLOGY,142,18-25. |
| MLA | Cai, Jinling,et al."Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5".BIORESOURCE TECHNOLOGY 142(2013):18-25. |
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| Screening and hydrog（533KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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