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Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence
Hao, Bin1,2; Mo, Zhao-Lan1,3; Xiao, Peng1; Pan, Hai-Jian1,2; Lan, Xin1,2; Li, Gui-Yang1,3; Mo, ZL
2013-03-01
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号97期号:6页码:2575-2585
文章类型Article
摘要The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.; The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.
关键词Vibrio Anguillarum Rpon (Sigma(54)) Biofilm Exopolysaccharides Metalloproteases Virulence
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s00253-012-4372-x
URL查看原文
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000315650100027
WOS关键词MEDIATED IRON UPTAKE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; CHEMOTACTIC MOTILITY ; HEMOLYSIN GENE ; UPTAKE SYSTEM ; METALLOPROTEASE ; IDENTIFICATION ; EXPRESSION ; COLONIZATION
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16621
专题实验海洋生物学重点实验室
通讯作者Mo, ZL
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,et al. Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2575-2585.
APA Hao, Bin.,Mo, Zhao-Lan.,Xiao, Peng.,Pan, Hai-Jian.,Lan, Xin.,...&Mo, ZL.(2013).Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2575-2585.
MLA Hao, Bin,et al."Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2575-2585.
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