Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential

doi:10.1016/j.jprot.2013.07.033

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	Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential
	Zhang, Jian 1,2; Hu, Yong-hua 1; Xiao, Zhi-zhong 1; Sun, Li 1; Sun, L
	2013-10-08
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	91 页码:430-443
文章类型	Article
摘要	Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection.; Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection. Biological significance This study provides the first proteomic picture of turbot in response to megalocytivirus infection. We demonstrated that megalocytivirus infection modulates the expression of turbot proteins associated with various cellular functions, and that one of the upregulated proteins, NKEF, exhibits antiviral effect when overexpressed in uiuo, while another upregulated protein, Hsp70, exhibits adjuvant effect when co-immunized with a DNA vaccine. These results add molecular insights into turbot immune response induced by megalocytivirus and provide candidate proteins with application potentials in the control of megalocytivirus-associated disease. (C) 2013 Elsevier B.V. All rights reserved.
关键词	Megalocytivirus Scophthalmus Maximus Proteomic Antiviral Adjuvant
学科领域	Biochemistry & Molecular Biology
DOI	10.1016/j.jprot.2013.07.033
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000327906000034
WOS关键词	RED-SEA BREAM ; FLOUNDER PARALICHTHYS-OLIVACEUS ; LARGE YELLOW CROAKER ; RECEPTOR-MEDIATED ENDOCYTOSIS ; TROUT ONCORHYNCHUS-MYKISS ; ANTIGEN-PRESENTING CELLS ; COMPLETE GENOME SEQUENCE ; IRIDOVIRUS-LIKE AGENT ; NATURAL-KILLER-CELLS ; IN-VITRO INHIBITION
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16602
专题	实验海洋生物学重点实验室
通讯作者	Sun, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Jian,Hu, Yong-hua,Xiao, Zhi-zhong,et al. Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential[J]. JOURNAL OF PROTEOMICS,2013,91:430-443.
APA	Zhang, Jian,Hu, Yong-hua,Xiao, Zhi-zhong,Sun, Li,&Sun, L.(2013).Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential.JOURNAL OF PROTEOMICS,91,430-443.
MLA	Zhang, Jian,et al."Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential".JOURNAL OF PROTEOMICS 91(2013):430-443.

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