Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China | |
| Li, Jialin1; Li, Fuchao2; Yu, Shuxian1,3; Qin, Song1; Wang, Guangyi4,5; Qin, S | |
| 2013-11-01 | |
| 发表期刊 | MICROBIAL ECOLOGY
 |
| ISSN | 0095-3628 |
| 卷号 | 66期号:4页码:861-870 |
| 文章类型 | Article |
| 摘要 | Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of gamma- and delta-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, gamma-Proteobacteria were dominant in all samples (21.7 similar to 45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 (3-)-P, NH4 (+)-N, and Chlorophyll a content. The phyla of gamma-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.; Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of gamma- and delta-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, gamma-Proteobacteria were dominant in all samples (21.7 similar to 45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 (3-)-P, NH4 (+)-N, and Chlorophyll a content. The phyla of gamma-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture. |
| 学科领域 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology |
| DOI | 10.1007/s00248-013-0272-6 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology |
| WOS类目 | Ecology ; Marine & Freshwater Biology ; Microbiology |
| WOS记录号 | WOS:000325778500014 |
| WOS关键词 | AMMONIA-OXIDIZING BACTERIA ; FISH FARM SEDIMENTS ; MICROBIAL COMMUNITY ; ENVIRONMENTAL GRADIENTS ; SPATIAL-DISTRIBUTION ; CHANGJIANG ESTUARY ; SURFACE SEDIMENTS ; CLONE LIBRARIES ; COASTAL WATERS ; SEA |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16594 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Qin, S |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China 5.Univ Hawaii Manoa, Dept Microbiol, Honolulu, HI 96822 USA |
| 推荐引用方式 GB/T 7714 | Li, Jialin,Li, Fuchao,Yu, Shuxian,et al. Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China[J]. MICROBIAL ECOLOGY,2013,66(4):861-870. |
| APA | Li, Jialin,Li, Fuchao,Yu, Shuxian,Qin, Song,Wang, Guangyi,&Qin, S.(2013).Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China.MICROBIAL ECOLOGY,66(4),861-870. |
| MLA | Li, Jialin,et al."Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China".MICROBIAL ECOLOGY 66.4(2013):861-870. |
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| Impacts of Maricultu（426KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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