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Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China
Liu, Haizhou1; Xiao, Lin1,2; Wei, Jianteng1; Schmitz, John C.3; Liu, Ming1; Wang, Cuicui1; Cheng, Linyou1; Wu, Ning1; Chen, Lei1; Zhang, Yuyan4; Lin, Xiukun1,5; Lin, XK
2013-07-01
发表期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
卷号29期号:7页码:1271-1278
文章类型Article
摘要A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents.; A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents.
关键词Moderately Halophilic Actinomycetes Streptomyces 8-o-methyltetrangulol Naphthomycin a
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s11274-013-1290-8
URL查看原文
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000321976800015
WOS关键词HALOPHILIC ACTINOMYCETE ; STRUCTURE ELUCIDATION ; BIOLOGICAL-ACTIVITY ; SEQUENCE ALIGNMENT ; NAPHTHOMYCIN ; ANSAMYCIN ; SEDIMENTS ; GENE ; TETRANGOMYCIN ; TETRANGULOL
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16591
专题实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
通讯作者Lin, XK
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China
3.Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA
4.Qingdao Univ Sci & Technol, Sch Chem Engn, Qingdao 266042, Shandong, Peoples R China
5.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
第一作者单位中国科学院海洋研究所
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Liu, Haizhou,Xiao, Lin,Wei, Jianteng,et al. Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(7):1271-1278.
APA Liu, Haizhou.,Xiao, Lin.,Wei, Jianteng.,Schmitz, John C..,Liu, Ming.,...&Lin, XK.(2013).Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(7),1271-1278.
MLA Liu, Haizhou,et al."Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.7(2013):1271-1278.
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