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Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics
Jiao, Shuang1,2; Nie, Miaomiao1,2,3; Song, Hongbin4; Xu, Dongdong4; You, Feng1,2
2020-05-01
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume715Pages:15
Corresponding AuthorXu, Dongdong(xudong0580@163.com) ; You, Feng(youfeng@qdio.ac.cn)
AbstractAs global climate changes, mass mortality in farmed fish associated with the severely cold weather has aroused growing concerns. Yellow drum (Nibea albijiora) is an important maricultured fish in China, whereby its aquaculture suffered from overwinter mortality associated with cold and cold-induced-fasting stresses. Here, by using LC-MS metabolomics combined with transcriptomics, we investigated the physiological responses of yellow drum liver to cold and starvation stresses. The experiment involved four groups: 16 degrees C fed group (CG1), 16 degrees C unfed group (CG2), 8 degrees C fed group (EG1), and 8 degrees C unfed group (EG2). Under cold stress, a total of 308 and 257 differential metabolites were identified in EG1 vs. CG 1 and EG2 vs. CG2, respectively, showing 5 overlapping significant pathways: glutathione metabolism, biosynthesis of unsaturated fatty adds, galactose metabolism, arginine and proline metabolism, and ABC transporters. Intersection analysis identified that glutamate, oxidized glutathione (GSSG), and eicosenoic acid were the common metabolites induced by cold stress. Under starvation stress, a total of 300 and 215 differential metabolites were identified in CG2 vs. CG1 and EG2 vs. EG1, respectively, showing 2 overlapping significant pathways: glutathione metabolism and galactose metabolism. Intersection analysis revealed that glutamate and GSSG were the common metabolites caused by fasting. Under cold and starvation combined stresses, 286 differential metabolites were identified in EG2 vs. CG1, showing 7 influenced pathways: glycerophospholipid metabolism, biosynthesis of unsaturated fatty acids, glutathione metabolism, sphingolipid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, autophagy, and purine metabolism. Interestingly, the glutamate and GSSG were induced by both single and combined stresses of cold and starvation treatments. These findings suggest that glutathione metabolism and its related metabolites (glutamate and GSSG) could be potential biomarkers of cold and starvation stresses in yellow drum. Overall, the results of this study provided insights into the physiological regulation in response to cold and starvation stresses in this fish. (C) 2020 Published by Elsevier B.V.
KeywordNibea albiflora Low temperature Starvation Metabolomics Liver
DOI10.1016/j.scitotenv.2020.136940
Indexed BySCI
Language英语
Funding ProjectProject of Zhejiang Province of China[2017C04003] ; National Natural Science Foundation of China[41476127] ; National Natural Science Foundation of China[31502146] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; Key Research and Development Program of Shandong Province, China[2019GHY112007]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000518409900110
PublisherELSEVIER
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165880
Collection实验海洋生物学重点实验室
Corresponding AuthorXu, Dongdong; You, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
4.Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiao, Shuang,Nie, Miaomiao,Song, Hongbin,et al. Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,715:15.
APA Jiao, Shuang,Nie, Miaomiao,Song, Hongbin,Xu, Dongdong,&You, Feng.(2020).Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics.SCIENCE OF THE TOTAL ENVIRONMENT,715,15.
MLA Jiao, Shuang,et al."Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics".SCIENCE OF THE TOTAL ENVIRONMENT 715(2020):15.
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