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Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment
Liu, Rui1; Qiu, Limei1; Yu, Zuoan3; Zi, Jun1; Yue, Feng1,2; Wang, Lingling1; Zhang, Huan1; Teng, Weiming3; Liu, Xiangfeng3; Song, Linsheng1; Song, LS
2013-10-01
发表期刊JOURNAL OF INVERTEBRATE PATHOLOGY
ISSN0022-2011
卷号114期号:2页码:144-150
文章类型Article
摘要A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.; A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.
关键词Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature
学科领域Zoology
DOI10.1016/j.jip.2013.07.005
URL查看原文
收录类别SCI
语种英语
WOS研究方向Zoology
WOS类目Zoology
WOS记录号WOS:000324790400006
WOS关键词BROWN RING DISEASE ; CLAM RUDITAPES-PHILIPPINARUM ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; SHELLFISH ; BACTERIA ; TAPETIS ; ALGINOLYTICUS ; MORTALITIES ; RESPONSES
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:87[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16586
专题实验海洋生物学重点实验室
通讯作者Song, LS
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Liaoning Ocean & Fisheries Sci Res Inst, Liaoning Key Lab Marine Fisheries Mol Biol, Dalian 116023, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Liu, Rui,Qiu, Limei,Yu, Zuoan,et al. Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2013,114(2):144-150.
APA Liu, Rui.,Qiu, Limei.,Yu, Zuoan.,Zi, Jun.,Yue, Feng.,...&Song, LS.(2013).Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment.JOURNAL OF INVERTEBRATE PATHOLOGY,114(2),144-150.
MLA Liu, Rui,et al."Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment".JOURNAL OF INVERTEBRATE PATHOLOGY 114.2(2013):144-150.
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