Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei

doi:10.1016/j.fsi.2012.09.016

IOCAS-IR > 实验海洋生物学重点实验室

	Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei
	Sun, Rui 1,2; Qiu, Limei 1; Yue, Feng 1,2; Wang, Lingling 1; Liu, Rui 1; Zhou, Zhi 1; Zhang, Huan 1; Song, Linsheng 1; Song, LS
	2013
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	34 期号:1 页码:38-45
文章类型	Article
摘要	CpG oligodeoxynucleotides (CpG ODNs), also called bacterial DNA or synthetic oligodeoxynucleotides, can induce apparent immunity protection against various pathogens, and they are widely used as functional immunostimulant or vaccine adjuvant in mammals. In the present study, CpG-rich plasmid pUC57-CpG was constructed and employed to stimulate the shrimp Litopenaeus vannamei, and the total hemocyte count, percentage of apoptotic hemocytes, regeneration of circulating hemocytes, the ability of phagocytosis and generation of reactive oxygen species (ROS) were measured to reveal the possible protection mechanism of CpG ODNs. After the injection of pUC57-CpG, the total hemocyte count significantly decreased (p < 0.01) to 2.56 x 10(7) cell/mL at the first day post stimulation, while the apoptosis increased (p < 0.01), which was 1.72-fold of that in control group. At the same time, the regeneration of circulating hemocytes fluctuated in a similar trend, and a significant increase was observed at the first day post stimulation. The phagocytotic activity including the percentage of phagocytosis and phagocytotic index, experienced an upward tend during the whole experimental period and the ROS level increased by 22% (p < 0.05) compared to that in the control group at first day post stimulation. These results together suggested that pUC57-CpG could promote the apoptosis and regeneration of circulating hemocytes, and enhance the phagocytosis and ROS production, which might contribute to the boosted immunity against the infection of pathogens. (c) 2012 Elsevier Ltd. All rights reserved.; CpG oligodeoxynucleotides (CpG ODNs), also called bacterial DNA or synthetic oligodeoxynucleotides, can induce apparent immunity protection against various pathogens, and they are widely used as functional immunostimulant or vaccine adjuvant in mammals. In the present study, CpG-rich plasmid pUC57-CpG was constructed and employed to stimulate the shrimp Litopenaeus vannamei, and the total hemocyte count, percentage of apoptotic hemocytes, regeneration of circulating hemocytes, the ability of phagocytosis and generation of reactive oxygen species (ROS) were measured to reveal the possible protection mechanism of CpG ODNs. After the injection of pUC57-CpG, the total hemocyte count significantly decreased (p < 0.01) to 2.56 x 10(7) cell/mL at the first day post stimulation, while the apoptosis increased (p < 0.01), which was 1.72-fold of that in control group. At the same time, the regeneration of circulating hemocytes fluctuated in a similar trend, and a significant increase was observed at the first day post stimulation. The phagocytotic activity including the percentage of phagocytosis and phagocytotic index, experienced an upward tend during the whole experimental period and the ROS level increased by 22% (p < 0.05) compared to that in the control group at first day post stimulation. These results together suggested that pUC57-CpG could promote the apoptosis and regeneration of circulating hemocytes, and enhance the phagocytosis and ROS production, which might contribute to the boosted immunity against the infection of pathogens. (c) 2012 Elsevier Ltd. All rights reserved.
关键词	Cpg Odn Litopenaeus Vannamei Apoptosis Hematopoiesis Phagocytosis
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.09.016
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000314743600005
WOS关键词	PROTEIN PAP GENE ; SIGNAL-TRANSDUCTION ; BACTERIAL-DNA ; IN-VIVO ; CELLS ; OLIGODEOXYNUCLEOTIDES ; PHAGOCYTOSIS ; HEMATOPOIESIS ; EXPRESSION ; RECEPTOR
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16583
专题	实验海洋生物学重点实验室
通讯作者	Song, LS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Rui,Qiu, Limei,Yue, Feng,et al. Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):38-45.
APA	Sun, Rui.,Qiu, Limei.,Yue, Feng.,Wang, Lingling.,Liu, Rui.,...&Song, LS.(2013).Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,34(1),38-45.
MLA	Sun, Rui,et al."Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):38-45.

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