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| Genetic diversity and relationships of the brown alga Undaria pinnatifida cultivated along the Dalian Coast as revealed by amplified fragment length polymorphism markers | |
| Li, Shiguo1,2; Qiao, Kun1; Shan, Tifeng3; Pang, Shaojun3; Hou, Hesheng1; Hou, HS | |
| 2013-08-01 | |
| 发表期刊 | JOURNAL OF APPLIED PHYCOLOGY
 |
| ISSN | 0921-8971 |
| 卷号 | 25期号:4页码:1255-1263 |
| 文章类型 | Article |
| 摘要 | In this study, the amplified fragment length polymorphism (AFLP) method was employed to estimate the genetic diversity of young sporophytes belonging to six Undaria pinnatifida populations collected from five seaweed cultivation farms along the Dalian Coast of China in 2008. A total of 397 loci were detected using ten combinations of selective primer pairs. Of these, 302 of which (76.07 %) were polymorphic, which was mainly caused by the 40 accessions of two populations collected from the LWT (44.08 %) and the JST (44.58 %) farms. According to the UPGMA dendrogram and biplot of principal component analysis, the 40 accessions represented high intra-population genetic diversity and far relationship from all of other populations. In contrast, the accessions from the other populations (HK, MS, LS, and QD) represented high intra-population similarity. The four populations may have originated from the same introduced U. pinnatifida strain. Analysis of molecular variance showed that a higher proportion of genetic variation resided among the populations (63.56 %) than that within populations (36.64 %). It is hypothesized that the differences in genetic traits, as well as the mixed mating system and reduced gene flow were the primary reasons that caused the high genetic variation among populations and the high similarity between accessions within the population. However, the genetic diversity of the partial U. pinnatifida cultivar populations in Dalian farms has been decreasing in the past years, which poses a potential risk of germplasm degradation in cultivation. In addition, the breeding techniques of cultivated U. pinnatifida in China and the application of AFLP in Undaria are discussed in this article. Based on the results, the identified polymorphic markers could be used for genetic improvement of the species through marker-assisted breeding and the results also provide excellent information for selecting indigenous gametophyte resources from Dalian farms using different breeding methods.; In this study, the amplified fragment length polymorphism (AFLP) method was employed to estimate the genetic diversity of young sporophytes belonging to six Undaria pinnatifida populations collected from five seaweed cultivation farms along the Dalian Coast of China in 2008. A total of 397 loci were detected using ten combinations of selective primer pairs. Of these, 302 of which (76.07 %) were polymorphic, which was mainly caused by the 40 accessions of two populations collected from the LWT (44.08 %) and the JST (44.58 %) farms. According to the UPGMA dendrogram and biplot of principal component analysis, the 40 accessions represented high intra-population genetic diversity and far relationship from all of other populations. In contrast, the accessions from the other populations (HK, MS, LS, and QD) represented high intra-population similarity. The four populations may have originated from the same introduced U. pinnatifida strain. Analysis of molecular variance showed that a higher proportion of genetic variation resided among the populations (63.56 %) than that within populations (36.64 %). It is hypothesized that the differences in genetic traits, as well as the mixed mating system and reduced gene flow were the primary reasons that caused the high genetic variation among populations and the high similarity between accessions within the population. However, the genetic diversity of the partial U. pinnatifida cultivar populations in Dalian farms has been decreasing in the past years, which poses a potential risk of germplasm degradation in cultivation. In addition, the breeding techniques of cultivated U. pinnatifida in China and the application of AFLP in Undaria are discussed in this article. Based on the results, the identified polymorphic markers could be used for genetic improvement of the species through marker-assisted breeding and the results also provide excellent information for selecting indigenous gametophyte resources from Dalian farms using different breeding methods. |
| 关键词 | Undaria Pinnatifida Phaeophyta Aflp Genetic Diversity Population Structure Seaweed Breeding |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10811-012-9922-1 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000321588800036 |
| WOS关键词 | MITOCHONDRIAL COX3 GENE ; GAMETOPHYTE CLONES ; MATING SYSTEM ; PHAEOPHYCEAE ; LAMINARIALES ; AFLP ; POPULATIONS ; SEQUENCES ; JAPONICA ; IDENTIFICATION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16576 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Hou, HS |
| 作者单位 | 1.Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China 2.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Harbin 150070, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shiguo,Qiao, Kun,Shan, Tifeng,et al. Genetic diversity and relationships of the brown alga Undaria pinnatifida cultivated along the Dalian Coast as revealed by amplified fragment length polymorphism markers[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(4):1255-1263. |
| APA | Li, Shiguo,Qiao, Kun,Shan, Tifeng,Pang, Shaojun,Hou, Hesheng,&Hou, HS.(2013).Genetic diversity and relationships of the brown alga Undaria pinnatifida cultivated along the Dalian Coast as revealed by amplified fragment length polymorphism markers.JOURNAL OF APPLIED PHYCOLOGY,25(4),1255-1263. |
| MLA | Li, Shiguo,et al."Genetic diversity and relationships of the brown alga Undaria pinnatifida cultivated along the Dalian Coast as revealed by amplified fragment length polymorphism markers".JOURNAL OF APPLIED PHYCOLOGY 25.4(2013):1255-1263. |
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