Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development

doi:10.1016/j.dci.2013.04.005

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	Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development
	Yue, Xin 1; Huan, Pin 1; Xiao, Guoqiang 2; Liu, Baozhong1 ; Liu, BZ
	2013-09-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	41 期号:1 页码:27-32
文章类型	Article
摘要	An i-type lysozyme (MmeLys) has been proved to function in immunity of the clam Meretrix meretrix in our previous studies. In this study, the expression patterns of MmeLys mRNA and protein at four chief developmental stages of M. meretrix were analyzed, which was able to provide information about how ontogeny of immunity and, in particular, antibacterial ability occured in the bivalve. The results of real-time PCR and western blot showed that MmeLys expressions were activated in D-veligers and dramatically increased to the highest level in pediveligers. It is proposed that the expression changes at these two stages might be due to the visceral organs changes, which were related to the archenteron formation in D-veligers and the organ-restructuring in pediveligers during metamorphosis. In addition, new methods of whole mount in situ hybridization and whole mount immunofluorescence were applied to identify the MmeLys expression tissues, and these tissues (i.e. hepatopancreas, gill, mantle, mouth, velum and foot) may be involved in the immune function during development of clams. Our study is valuable to a certain extent for exploring the origin of immune functions in clams and provides new methodology for future studies on the immune ontogeny of bivalves. (C) 2013 Elsevier Ltd. All rights reserved.; An i-type lysozyme (MmeLys) has been proved to function in immunity of the clam Meretrix meretrix in our previous studies. In this study, the expression patterns of MmeLys mRNA and protein at four chief developmental stages of M. meretrix were analyzed, which was able to provide information about how ontogeny of immunity and, in particular, antibacterial ability occured in the bivalve. The results of real-time PCR and western blot showed that MmeLys expressions were activated in D-veligers and dramatically increased to the highest level in pediveligers. It is proposed that the expression changes at these two stages might be due to the visceral organs changes, which were related to the archenteron formation in D-veligers and the organ-restructuring in pediveligers during metamorphosis. In addition, new methods of whole mount in situ hybridization and whole mount immunofluorescence were applied to identify the MmeLys expression tissues, and these tissues (i.e. hepatopancreas, gill, mantle, mouth, velum and foot) may be involved in the immune function during development of clams. Our study is valuable to a certain extent for exploring the origin of immune functions in clams and provides new methodology for future studies on the immune ontogeny of bivalves. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Meretrix Meretrix Lysozyme Larval Immunity Whole Mount In Situ Hybridization Whole Mount Immunofluorescence
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2013.04.005
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000321091400004
WOS关键词	PENAEUS-SETIFERUS DECAPODA ; OYSTER CRASSOSTREA-GIGAS ; INNATE IMMUNE-RESPONSE ; GENE-EXPRESSION ; BIVALVE MOLLUSKS ; EASTERN OYSTER ; MANDUCA-SEXTA ; CATHEPSIN-B ; METAMORPHOSIS ; ONTOGENY
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16571
专题	实验海洋生物学重点实验室
通讯作者	Liu, BZ
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yue, Xin,Huan, Pin,Xiao, Guoqiang,et al. Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(1):27-32.
APA	Yue, Xin,Huan, Pin,Xiao, Guoqiang,Liu, Baozhong,&Liu, BZ.(2013).Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(1),27-32.
MLA	Yue, Xin,et al."Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.1(2013):27-32.

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