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Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
Bao, Y. B.1; Li, L.2; Ye, M. X.1; Dong, Y. H.1; Jin, W. X.1; Lin, Z. H.1; Lin, ZH
2013
发表期刊GENETICS AND MOLECULAR RESEARCH
ISSN1676-5680
卷号12期号:2页码:1143-1154
文章类型Article
摘要The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration.; The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration.
关键词Vibrio Parahaemolyticus Messenger Rna Expression Immune Response Tegillarca Granosa Glutamine Synthetase
学科领域Biochemistry & Molecular Biology ; Genetics & Heredity
DOI10.4238/2013.April.10.9
URL查看原文
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000320030100028
WOS关键词EVOLUTIONARY RELATIONSHIPS ; GENE ; CLONING ; CDNA ; ENCODE
WOS标题词Science & Technology ; Life Sciences & Biomedicine
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16570
专题实验海洋生物学重点实验室
通讯作者Lin, ZH
作者单位1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
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Bao, Y. B.,Li, L.,Ye, M. X.,et al. Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(2):1143-1154.
APA Bao, Y. B..,Li, L..,Ye, M. X..,Dong, Y. H..,Jin, W. X..,...&Lin, ZH.(2013).Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides.GENETICS AND MOLECULAR RESEARCH,12(2),1143-1154.
MLA Bao, Y. B.,et al."Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides".GENETICS AND MOLECULAR RESEARCH 12.2(2013):1143-1154.
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