Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai

doi:10.1016/j.fsi.2013.02.025

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	Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai
	Qiu, Reng 1,2; Liu, Xiao 1; Hu, Yong-hua 1; Sun, Bo-guang1 ; Sun, BG
	2013-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	34 期号:5 页码:1376-1382
文章类型	Article
摘要	Cathepsin B (EC 3.4.22.1) is a member of lysosomal cysteine protease and has a papain-like fold. In mammals, it is involved in protein degradation and other physiological processes including immune response. However, little is known about the function of cathepsin B in mollusks. In this study, we identified and analyzed a cathepsin B homolog (HdCatB) from Pacific abalone (Haliotis discus hannai), an economically important mollusk species cultured in East Asia. HdCatB is composed of 336 amino acid residues and its mature form is predicted to start at residue 86. HdCatB possesses typical domain architecture of cathepsin B and contains a propeptide region and a cysteine protease domain, the latter containing the four active site residues (Q108, C114, H282, and N302) that are conserved in many different organisms. HdCatB shares 40-60% overall sequence identities with the cathepsin Bofa number of vertebrates and invertebrates and is phylogenetically very close to mollusk cathepsin B. Quantitative real time RT-PCR analysis revealed that HdCatB expression occurred in multiple tissues and was upregulated by bacterial infection. Recombinant HdCatB purified from Escherichia coli exhibited apparent protease activity, which was optimal at 45 degrees C and pH 6.0. These results indicate that HdCatB is a bioactive protease that is likely to be implicated in the immune response of abalone during bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.; Cathepsin B (EC 3.4.22.1) is a member of lysosomal cysteine protease and has a papain-like fold. In mammals, it is involved in protein degradation and other physiological processes including immune response. However, little is known about the function of cathepsin B in mollusks. In this study, we identified and analyzed a cathepsin B homolog (HdCatB) from Pacific abalone (Haliotis discus hannai), an economically important mollusk species cultured in East Asia. HdCatB is composed of 336 amino acid residues and its mature form is predicted to start at residue 86. HdCatB possesses typical domain architecture of cathepsin B and contains a propeptide region and a cysteine protease domain, the latter containing the four active site residues (Q108, C114, H282, and N302) that are conserved in many different organisms. HdCatB shares 40-60% overall sequence identities with the cathepsin Bofa number of vertebrates and invertebrates and is phylogenetically very close to mollusk cathepsin B. Quantitative real time RT-PCR analysis revealed that HdCatB expression occurred in multiple tissues and was upregulated by bacterial infection. Recombinant HdCatB purified from Escherichia coli exhibited apparent protease activity, which was optimal at 45 degrees C and pH 6.0. These results indicate that HdCatB is a bioactive protease that is likely to be implicated in the immune response of abalone during bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Cathepsin b Mollusk Abalone Haliotis Discus Cysteine Protease
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2013.02.025
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000317870600046
WOS关键词	LYSOSOMAL CYSTEINE PROTEASES ; CELL-DEATH ; IDENTIFICATION ; PURIFICATION ; OYSTER ; INHIBITORS ; DISEASES ; COMPLEX ; STRAIN ; GENES
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16569
专题	实验海洋生物学重点实验室
通讯作者	Sun, BG
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Qiu, Reng,Liu, Xiao,Hu, Yong-hua,et al. Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1376-1382.
APA	Qiu, Reng,Liu, Xiao,Hu, Yong-hua,Sun, Bo-guang,&Sun, BG.(2013).Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,34(5),1376-1382.
MLA	Qiu, Reng,et al."Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1376-1382.

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