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Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes
He, Linwen1,2; Zhang, Xiaojuan1,2; Wang, Guangce1; Wang, GC
2013-07-01
发表期刊PHYCOLOGICAL RESEARCH
ISSN1322-0829
卷号61期号:3页码:172-179
文章类型Article
摘要Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes.; Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes.
关键词C-4 Phosphoenolpyruvate Carboxykinase Porphyra Haitanensis Quantitative Polymerase Chain Reaction Rapid Amplification Of Cdna Ends
学科领域Marine & Freshwater Biology
DOI10.1111/pre.12005
URL查看原文
收录类别SCI
语种英语
WOS研究方向Marine & Freshwater Biology
WOS类目Marine & Freshwater Biology
WOS记录号WOS:000321753200002
WOS关键词C-4 PHOTOSYNTHESIS ; DNA-SEQUENCE ; RED ALGA ; GENE ; GLUCONEOGENESIS ; CHENOPODIACEAE ; PURIFICATION ; METABOLISM ; MECHANISM ; EVOLUTION
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16567
专题实验海洋生物学重点实验室
通讯作者Wang, GC
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所
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He, Linwen,Zhang, Xiaojuan,Wang, Guangce,et al. Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes[J]. PHYCOLOGICAL RESEARCH,2013,61(3):172-179.
APA He, Linwen,Zhang, Xiaojuan,Wang, Guangce,&Wang, GC.(2013).Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes.PHYCOLOGICAL RESEARCH,61(3),172-179.
MLA He, Linwen,et al."Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes".PHYCOLOGICAL RESEARCH 61.3(2013):172-179.
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