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Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes | |
He, Linwen1,2; Zhang, Xiaojuan1,2; Wang, Guangce1; Wang, GC | |
2013-07-01 | |
发表期刊 | PHYCOLOGICAL RESEARCH |
ISSN | 1322-0829 |
卷号 | 61期号:3页码:172-179 |
文章类型 | Article |
摘要 | Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes.; Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes. |
关键词 | C-4 Phosphoenolpyruvate Carboxykinase Porphyra Haitanensis Quantitative Polymerase Chain Reaction Rapid Amplification Of Cdna Ends |
学科领域 | Marine & Freshwater Biology |
DOI | 10.1111/pre.12005 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Marine & Freshwater Biology |
WOS类目 | Marine & Freshwater Biology |
WOS记录号 | WOS:000321753200002 |
WOS关键词 | C-4 PHOTOSYNTHESIS ; DNA-SEQUENCE ; RED ALGA ; GENE ; GLUCONEOGENESIS ; CHENOPODIACEAE ; PURIFICATION ; METABOLISM ; MECHANISM ; EVOLUTION |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/16567 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Wang, GC |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | He, Linwen,Zhang, Xiaojuan,Wang, Guangce,et al. Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes[J]. PHYCOLOGICAL RESEARCH,2013,61(3):172-179. |
APA | He, Linwen,Zhang, Xiaojuan,Wang, Guangce,&Wang, GC.(2013).Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes.PHYCOLOGICAL RESEARCH,61(3),172-179. |
MLA | He, Linwen,et al."Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes".PHYCOLOGICAL RESEARCH 61.3(2013):172-179. |
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