Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum | |
Wang, Chong1,2; Hu, Yong-hua1; Sun, Bo-guang1 ; Chi, Heng1; Li, Jun1,3; Sun, Li1; Sun, L
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| 2013-03-13 | |
| 发表期刊 | DISEASES OF AQUATIC ORGANISMS
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| ISSN | 0177-5103 |
| 卷号 | 103期号:1页码:45-53 |
| 文章类型 | Article |
| 摘要 | Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to turbot Scophthalmus maximus via immersion and oral feeding, P1SW and V3SW at a dose of 2 x 10(8) colony-forming units caused no mortality, but both strains were able to disseminate into internal organs in a transient, time-dependent manner. When turbot were immunized with P1SW, V3SW, or P1SW plus V3SW (named P1V3) via immersion plus oral routes, the latter with vaccines embedded in sodium alginate microspheres, moderate protection against E. tarda and V. anguillarum was induced by V3SW, and moderate protection against E. tarda was induced by P1SW. Compared to P1SW and V3SW, P1V3 elicited a significantly stronger protection against both E. tarda and V. anguillarum. Immunological analysis showed that (1) P1SW, V3SW, and especially P1V3 activated head kidney macrophages, (2) P1V3 induced significantly higher levels of serum antibodies against E. tarda and V. anguillarum than P1SW and V3SW, and (3) P1V3-induced antibodies were able to bind E. tarda and V. anguillarum and enhance serum bactericidal activity. These results indicate that P1V3 as a naturally delivered vaccine elicited a humoral immune response against both E. tarda and V. anguillarum and, as a result, was cross-protective against E. tarda and V. anguillarum infection.; Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to turbot Scophthalmus maximus via immersion and oral feeding, P1SW and V3SW at a dose of 2 x 10(8) colony-forming units caused no mortality, but both strains were able to disseminate into internal organs in a transient, time-dependent manner. When turbot were immunized with P1SW, V3SW, or P1SW plus V3SW (named P1V3) via immersion plus oral routes, the latter with vaccines embedded in sodium alginate microspheres, moderate protection against E. tarda and V. anguillarum was induced by V3SW, and moderate protection against E. tarda was induced by P1SW. Compared to P1SW and V3SW, P1V3 elicited a significantly stronger protection against both E. tarda and V. anguillarum. Immunological analysis showed that (1) P1SW, V3SW, and especially P1V3 activated head kidney macrophages, (2) P1V3 induced significantly higher levels of serum antibodies against E. tarda and V. anguillarum than P1SW and V3SW, and (3) P1V3-induced antibodies were able to bind E. tarda and V. anguillarum and enhance serum bactericidal activity. These results indicate that P1V3 as a naturally delivered vaccine elicited a humoral immune response against both E. tarda and V. anguillarum and, as a result, was cross-protective against E. tarda and V. anguillarum infection. |
| 关键词 | Bivalent Vaccine Live Vaccine Natural Delivery Cross-protection |
| 学科领域 | Fisheries ; Veterinary Sciences |
| DOI | 10.3354/dao02569 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Veterinary Sciences |
| WOS类目 | Fisheries ; Veterinary Sciences |
| WOS记录号 | WOS:000316006700005 |
| WOS关键词 | OUTER-MEMBRANE PROTEINS ; FISH ; INFECTION ; STRAIN ; MECHANISMS ; EXPRESSION ; ANTIGEN |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16565 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Sainte Marie, MI 49783 USA |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Chong,Hu, Yong-hua,Sun, Bo-guang,et al. Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum[J]. DISEASES OF AQUATIC ORGANISMS,2013,103(1):45-53. |
| APA | Wang, Chong.,Hu, Yong-hua.,Sun, Bo-guang.,Chi, Heng.,Li, Jun.,...&Sun, L.(2013).Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum.DISEASES OF AQUATIC ORGANISMS,103(1),45-53. |
| MLA | Wang, Chong,et al."Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum".DISEASES OF AQUATIC ORGANISMS 103.1(2013):45-53. |
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