IOCAS-IR  > 实验海洋生物学重点实验室
Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis
Wang, Lijuan1,2; Wu, Zhihao1,2; Wang, Yingjun3; Liu, Mengxia3; Song, Aihuan3; Liu, Hongjun3; You, Feng1,2
2020-02-27
Source PublicationFRONTIERS IN MARINE SCIENCE
Volume7Pages:11
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractThe black rockfish Sebastes schlegelii is a commercially important fish species for marine fishery stock enhancement in Asia. This work aimed to evaluate the potential genetic impacts of releasing hatchery-reared juvenile black rockfish on wild stock in Lidao Bay, China. A partial sequence of the mitochondrial DNA (mtDNA) control region and 38 microsatellite DNA loci were used to assess the genetic impact. The haplotype diversity parameter (h) and nucleotide diversity parameter (pi) in hatchery-released stock were 0.902 and 0.00483, respectively. The h values in wild stock before stock enhancement and the mixed stock after enhancement were 0.970 and 0.939 (p = 0.025), respectively, and the pi values were 0.00581 and 0.00526 (p = 0.150), respectively. The mean effective number of alleles (Ae) in hatchery-released stock was 4.76, the mean polymorphism information content (PIC) was 0.674, the observed heterozygosity (Ho) was 0.668, and the expected heterozygosity (He) was 0.697. In wild stock before stock enhancement and the mixed stock after enhancement, the Ae, PIC, Ho, and He values were 6.01 and 5.82, 0.698 and 0.716, 0.709 and 0.741, and 0.735 and 0.754, respectively. These results indicated no marked decrease in the hatchery-released stock, although it displayed slightly lower levels of genetic diversity and heterozygosity than the wild stock. And the mixed stock after release exhibited similar genetic diversity to that of the wild stock before release. Accordingly, we propose that stock enhancement may not cause genetic diversity reduction on wild S. schlegelii stock in Lidao Bay over the short term. Whereas, discriminant analysis of principal components (DAPC) analysis identified three major clusters. The fixation index (F-ST) and analysis of molecular variance (AMOVA) also clearly showed low level but significant differentiations between the hatchery-released and wild stocks, and the wild stock before enhancement and the mixed stock after enhancement. Consequently, long-term genetic evaluation might be required.
KeywordSebastes schlegelii mitochondrial DNA microsatellite DNA genetic variation stock enhancement Lidao Bay
DOI10.3389/fmars.2020.00094
Indexed BySCI
Language英语
Funding ProjectMajor Agricultural Application Technology and Innovation Projects of Shandong Province (2017-2020) ; Special Program for Basic Research of the Ministry of Science and Technology of China[2014FY110500] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000517573800001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165629
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Marine Biol Inst Shandong Prov, Qingdao, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Lijuan,Wu, Zhihao,Wang, Yingjun,et al. Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis[J]. FRONTIERS IN MARINE SCIENCE,2020,7:11.
APA Wang, Lijuan.,Wu, Zhihao.,Wang, Yingjun.,Liu, Mengxia.,Song, Aihuan.,...&You, Feng.(2020).Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis.FRONTIERS IN MARINE SCIENCE,7,11.
MLA Wang, Lijuan,et al."Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis".FRONTIERS IN MARINE SCIENCE 7(2020):11.
Files in This Item:
File Name/Size DocType Version Access License
Genetic Assessment o(1871KB)期刊论文出版稿开放获取CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Lijuan]'s Articles
[Wu, Zhihao]'s Articles
[Wang, Yingjun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Lijuan]'s Articles
[Wu, Zhihao]'s Articles
[Wang, Yingjun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Lijuan]'s Articles
[Wu, Zhihao]'s Articles
[Wang, Yingjun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.