Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary

doi:10.1093/dnares/dsz023

IOCAS-IR > 实验海洋生物学重点实验室

	Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary
	Liu, Qinghua 1,2,3; Wang, Xueying1,2,3 ; Xiao, Yongshuang 1,2,3; Zhao, Haixia1,2,3,4 ; Xu, Shihong 1,2,3; Wang, Yanfeng 1,2,3; Wu, Lele 1,2,3,4; Zhou, Li 1,2,3,4; Du, Tengfei 1,2,3,4; Lv, Xuejiao 1,2,3,4; Li, Jun 1,2,3
	2019-12-01
发表期刊	DNA RESEARCH
ISSN	1340-2838
卷号	26 期号:6 页码:12
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	Black rockfish (Sebastes schlegelii) is an economically important viviparous marine teleost in Japan, Korea, and China. It is characterized by internal fertilization, long-term sperm storage in the female ovary, and a high abortion rate. For better understanding the mechanism of fertilization and gestation, it is essential to establish a reference genome for viviparous teleosts. Herein, we used a combination of Pacific Biosciences sequel, Illumina sequencing platforms, 10x Genomics, and Hi-C technology to obtain a genome assembly size of 848.31Mb comprising 24 chromosomes, and contig and scaffold N50 lengths of 2.96 and 35.63Mb, respectively. We predicted 39.98% repetitive elements, and 26,979 protein-coding genes. S. schlegelii diverged from Gasterosteus aculeatus similar to 32.1-56.8 million years ago. Furthermore, sperm remained viable within the ovary for up to 6months. The glucose transporter SLC2 showed significantly positive genomic selection, and carbohydrate metabolism-related KEGG pathways were significantly up-regulated in ovaries after copulation. In vitro suppression of glycolysis with sodium iodoacetate reduced sperm longevity significantly. The results indicated the importance of carbohydrates in maintaining sperm survivability. Decoding the S. schlegelii genome not only provides new insights into sperm storage; additionally, it is highly valuable for marine researchers and reproduction biologists.
关键词	Sebastes schlegelii viviparous PacBio sequencing Hi-C genome assemble sperm storage
DOI	10.1093/dnares/dsz023
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901205] ; National Key R&D Program of China[2018YFD0901204] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31802278] ; China Agriculture Research System[CARS-47] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0302-4] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0302-5] ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000514627800002
出版者	OXFORD UNIV PRESS
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/165578
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Qinghua,Wang, Xueying,Xiao, Yongshuang,et al. Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary[J]. DNA RESEARCH,2019,26(6):12.
APA	Liu, Qinghua.,Wang, Xueying.,Xiao, Yongshuang.,Zhao, Haixia.,Xu, Shihong.,...&Li, Jun.(2019).Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary.DNA RESEARCH,26(6),12.
MLA	Liu, Qinghua,et al."Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary".DNA RESEARCH 26.6(2019):12.

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