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Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary
Liu, Qinghua1,2,3; Wang, Xueying1,2,3; Xiao, Yongshuang1,2,3; Zhao, Haixia1,2,3,4; Xu, Shihong1,2,3; Wang, Yanfeng1,2,3; Wu, Lele1,2,3,4; Zhou, Li1,2,3,4; Du, Tengfei1,2,3,4; Lv, Xuejiao1,2,3,4; Li, Jun1,2,3
2019-12-01
Source PublicationDNA RESEARCH
ISSN1340-2838
Volume26Issue:6Pages:12
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractBlack rockfish (Sebastes schlegelii) is an economically important viviparous marine teleost in Japan, Korea, and China. It is characterized by internal fertilization, long-term sperm storage in the female ovary, and a high abortion rate. For better understanding the mechanism of fertilization and gestation, it is essential to establish a reference genome for viviparous teleosts. Herein, we used a combination of Pacific Biosciences sequel, Illumina sequencing platforms, 10x Genomics, and Hi-C technology to obtain a genome assembly size of 848.31Mb comprising 24 chromosomes, and contig and scaffold N50 lengths of 2.96 and 35.63Mb, respectively. We predicted 39.98% repetitive elements, and 26,979 protein-coding genes. S. schlegelii diverged from Gasterosteus aculeatus similar to 32.1-56.8 million years ago. Furthermore, sperm remained viable within the ovary for up to 6months. The glucose transporter SLC2 showed significantly positive genomic selection, and carbohydrate metabolism-related KEGG pathways were significantly up-regulated in ovaries after copulation. In vitro suppression of glycolysis with sodium iodoacetate reduced sperm longevity significantly. The results indicated the importance of carbohydrates in maintaining sperm survivability. Decoding the S. schlegelii genome not only provides new insights into sperm storage; additionally, it is highly valuable for marine researchers and reproduction biologists.
KeywordSebastes schlegelii viviparous PacBio sequencing Hi-C genome assemble sperm storage
DOI10.1093/dnares/dsz023
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0901205] ; National Key R&D Program of China[2018YFD0901204] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31802278] ; China Agriculture Research System[CARS-47] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0302-4] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0302-5] ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000514627800002
PublisherOXFORD UNIV PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165578
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Qinghua,Wang, Xueying,Xiao, Yongshuang,et al. Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary[J]. DNA RESEARCH,2019,26(6):12.
APA Liu, Qinghua.,Wang, Xueying.,Xiao, Yongshuang.,Zhao, Haixia.,Xu, Shihong.,...&Li, Jun.(2019).Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary.DNA RESEARCH,26(6),12.
MLA Liu, Qinghua,et al."Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary".DNA RESEARCH 26.6(2019):12.
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