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The novel fish miRNA pol-miR-novel_171 and its target gene FAM49B play a critical role in apoptosis and bacterial infection
Li, Wen-rui1,2,3; Guan, Xiao-lu1,2; Jiang, Shuai1,2; Sun, Li1,2
2020-05-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号106页码:10
通讯作者Sun, Li(lsun@qdio.ac.cn)
摘要MicroRNAs (miRNAs) are a type of small, non-coding RNAs that participate in many cellular and biological processes by regulating mRNA stability. In a previous study, we identified 96 Japanese flounder (Paralichthys olivaceus) miRNAs responsive to the infection of Edwardsiella tarda, a bacterial pathogen to fish as well as humans. In the current study, we examined the regulation and function of one novel miRNA, i.e., pol-miR-novel_171, from the above 96 miRNA pool. We found that pol-miR-novel_171 expression was regulated by E. tarda and megalocytivirus in a pathogen-specific manner, and that pol-miR-novel_171 targeted the gene of FAM49B (family with sequence similarity 49 member B) of flounder (named PoFAM49B) by negative interaction with the 3'-UTR of PoFAM49B. To date, the function fish FAM49B is unknown. We found that PoFAM49B expressed in multiple tissues of flounder, and recombinant PoFAM49B interacted with and inhibited the growth of Gram-negative bacterial pathogens. Interference with PoFAM49B expression in flounder cells promoted E. tarda infection. Similar effects on E. tarda infection were observed with pol-miR-novel_171 overexpression. Consistently, in vivo knockdown of PoFAM49B in flounder enhanced E. tarda dissemination in fish tissues. Furthermore, interference with PoFAM49B expression, or overexpression of pol-miR-novel_171, promoted apoptosis of flounder cells, while in vitro and in vivo knockdown of PoFAM49B augmented the expressions of key apoptosis-associated genes. These results revealed for the first time the immune function of fish FAM49B and the regulatory mechanism of a novel fish miRNA by demonstrating that pol-miR-novel_171, via PoFAM49B, played a critical role in apoptosis and anti-bacterial immunity.
关键词Paralichthys olivaceus Edwardsiella tarda microRNA Family with sequence similarity 49 Apoptosis Infection Immune defense
DOI10.1016/j.dci.2020.103616
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31730100] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key R&D Program of China[2018YFD0900500] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS研究方向Fisheries ; Immunology ; Zoology
WOS类目Fisheries ; Immunology ; Zoology
WOS记录号WOS:000517853000017
出版者ELSEVIER SCI LTD
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/165575
专题实验海洋生物学重点实验室
通讯作者Sun, Li
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋大科学研究中心
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Li, Wen-rui,Guan, Xiao-lu,Jiang, Shuai,et al. The novel fish miRNA pol-miR-novel_171 and its target gene FAM49B play a critical role in apoptosis and bacterial infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,106:10.
APA Li, Wen-rui,Guan, Xiao-lu,Jiang, Shuai,&Sun, Li.(2020).The novel fish miRNA pol-miR-novel_171 and its target gene FAM49B play a critical role in apoptosis and bacterial infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,106,10.
MLA Li, Wen-rui,et al."The novel fish miRNA pol-miR-novel_171 and its target gene FAM49B play a critical role in apoptosis and bacterial infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 106(2020):10.
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