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| Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae) | |
Yang, Xiaoqi1,2,3; Li, Lu1,2,3 ; Wang, Xiuliang1,2; Yao, Jianting1,2; Duan, Delin1,2
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| 2020 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 卷号 | 21期号:1页码:15 |
| 通讯作者 | Duan, Delin(dlduan@qdio.ac.cn) |
| 摘要 | CRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of Saccharina japonica is still unclear. After cloning the full-length of CRY-DASH from S. japonica (sjCRY-DASH), we deduced that its open reading frame was 1779 bp long and encoded 592 amino acids. sjCRY-DASH transcription was rapidly upregulated within 30 min in response to blue light and exhibited 24 h periodicity with different photoperiods. Moreover, sjCRY-DASH maintained the same periodicity in suitable growth temperature, suggesting a close relationship between this periodicity and circadian rhythm regulation. Novel-m3234-5p, which was targeted to sjCRY-DASH, decreased with increasing sjCRY-DASH transcription, acting as a negative modulator of sjCRY-DASH. Six long non-coding RNAs classified as long intergenic non-coding RNAs (lincRNAs) exhibited co-expression with sjCRY-DASH. A miRNA sjCRY DASH lincRNA network was consequently identified. By predicting the endogenous competing mRNAs of novel-m3234-5p, we found that sjCRY-DASH indirectly participated in the regulation of DNA damage repair, protein synthesis and processing, and actin transport. In conclusion, our results revealed that non-coding RNAs participate in the regulation of sjCRY-DASH, which played vital roles in the growth and early development of S. japonica. |
| 关键词 | blue light circadian rhythm CRY-DASH non-coding RNA growth Saccharina japonica |
| DOI | 10.3390/ijms21010309 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Shandong Key Sci-Technology Research Project[2018SDKJ0502-1] ; National Natural Science Foundation of China[31772848] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000515378000309 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/165572 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Xiaoqi,Li, Lu,Wang, Xiuliang,et al. Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(1):15. |
| APA | Yang, Xiaoqi,Li, Lu,Wang, Xiuliang,Yao, Jianting,&Duan, Delin.(2020).Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(1),15. |
| MLA | Yang, Xiaoqi,et al."Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.1(2020):15. |
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| Non-Coding RNAs Part（4642KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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