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Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis
Yue, Xin1,2; Zhang, Shujing1,3; Yu, Jiajia1,3; Liu, Baozhong1,2,3
2019
发表期刊AQUATIC BIOLOGY
ISSN1864-7790
卷号28页码:55-65
通讯作者Liu, Baozhong(bzliu@qdio.ac.cn)
摘要Tanning is a complex extracellular process that is a mechanism for stabilizing proteinaceous extracellular structures. Phenoloxidases play important roles in cross-linking during tanning, and laccase (EC 1.10.3.2) is a member of the phenoloxidase enzyme class. In this study, we identified a laccase gene (MpLac) from the clam Meretrix petechialis and found that MpLac might be involved in shell periostracal tanning of clams. Using whole-mount in situ hybridization, we found that MpLac mRNA in the larval clam was mainly expressed in the mantle edge. In the adult clam, our quantitative real-time PCR results showed that the mantle was also a tissue with a high MpLac expression level; in addition, by combining the results of fluorescence in situ hybridization, H&E staining and transmission electron microscopy, we found that the inner epithelium of the outer fold of the mantle edge, which is involved in periostracum formation, was the exact region in which MpLac mRNA was expressed. Furthermore, knocking down the expression of MpLac by RNA interference (RNAi) bleached the new shell periostracum. All of our results suggest the involvement of MpLac in shell periostracal tanning of M. petechialis.
关键词Meretrix petechialis Laccase Shell periostracum Tanning
DOI10.3354/ab00709
收录类别SCI
语种英语
资助项目China Agriculture Research System[CARS-49] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology[YQ2018NO07] ; National Natural Science Foundation of China[NSFC 31772845]
WOS研究方向Marine & Freshwater Biology
WOS类目Marine & Freshwater Biology
WOS记录号WOS:000518432200004
出版者INTER-RESEARCH
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/165548
专题实验海洋生物学重点实验室
通讯作者Liu, Baozhong
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋大科学研究中心
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GB/T 7714
Yue, Xin,Zhang, Shujing,Yu, Jiajia,et al. Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis[J]. AQUATIC BIOLOGY,2019,28:55-65.
APA Yue, Xin,Zhang, Shujing,Yu, Jiajia,&Liu, Baozhong.(2019).Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis.AQUATIC BIOLOGY,28,55-65.
MLA Yue, Xin,et al."Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis".AQUATIC BIOLOGY 28(2019):55-65.
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