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Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis
Yue, Xin1,2; Zhang, Shujing1,3; Yu, Jiajia1,3; Liu, Baozhong1,2,3
2019
Source PublicationAQUATIC BIOLOGY
ISSN1864-7790
Volume28Pages:55-65
Corresponding AuthorLiu, Baozhong(bzliu@qdio.ac.cn)
AbstractTanning is a complex extracellular process that is a mechanism for stabilizing proteinaceous extracellular structures. Phenoloxidases play important roles in cross-linking during tanning, and laccase (EC 1.10.3.2) is a member of the phenoloxidase enzyme class. In this study, we identified a laccase gene (MpLac) from the clam Meretrix petechialis and found that MpLac might be involved in shell periostracal tanning of clams. Using whole-mount in situ hybridization, we found that MpLac mRNA in the larval clam was mainly expressed in the mantle edge. In the adult clam, our quantitative real-time PCR results showed that the mantle was also a tissue with a high MpLac expression level; in addition, by combining the results of fluorescence in situ hybridization, H&E staining and transmission electron microscopy, we found that the inner epithelium of the outer fold of the mantle edge, which is involved in periostracum formation, was the exact region in which MpLac mRNA was expressed. Furthermore, knocking down the expression of MpLac by RNA interference (RNAi) bleached the new shell periostracum. All of our results suggest the involvement of MpLac in shell periostracal tanning of M. petechialis.
KeywordMeretrix petechialis Laccase Shell periostracum Tanning
DOI10.3354/ab00709
Indexed BySCI
Language英语
Funding ProjectChina Agriculture Research System[CARS-49] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology[YQ2018NO07] ; National Natural Science Foundation of China[NSFC 31772845]
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000518432200004
PublisherINTER-RESEARCH
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165548
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Baozhong
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yue, Xin,Zhang, Shujing,Yu, Jiajia,et al. Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis[J]. AQUATIC BIOLOGY,2019,28:55-65.
APA Yue, Xin,Zhang, Shujing,Yu, Jiajia,&Liu, Baozhong.(2019).Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis.AQUATIC BIOLOGY,28,55-65.
MLA Yue, Xin,et al."Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis".AQUATIC BIOLOGY 28(2019):55-65.
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