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The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses
Huo, Da1,2,3,4; Sun, Lina1,2,3,4; Storey, Kenneth B.5; Zhang, Libin1,2,3,4; Liu, Shilin1,2,3,4; Sun, Jingchun1,2,3,4; Yang, Hongsheng1,2,3,4
2020-03-20
发表期刊SCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
卷号709页码:15
通讯作者Sun, Lina(sunlina@qdio.ac.cn) ; Yang, Hongsheng(hshyang@ms.qdio.ac.cn)
摘要The aquatic environment can be greatly impacted by thermal and hypoxic stresses, particularly caused by intensified global warming. Hence, there is an urgency to understand the response mechanisms of marine organisms to adverse environment. Although long non-coding RNAs (lncRNAs) are involved in many biological processes, their roles in stress responses still remain unclear. Here, differentially expressed (DE) lncRNAs and mRNAs were identified as responses to environmental stresses in the economically important sea cucumber, Apostichopus japonicus, and their potential roles were explored. Based on a total of 159, 355 and 495 significantly upregulated genes and 230, 518 and 647 significantly downregulated genes identified in the thermal, hypoxic and combination thermal + hypoxic stress treatments, respectively, we constructed DE-lncRNA-mRNA coexpression networks. Among the networks, eight shared pairs were identified from the three treatments, and based on the connectivity degree, MSTRG.27265, MSTRG.19729 and MSTRG.95524 were shown to be crucial lncRNAs. Among all the significantly changed lncRNAs identified by RT-qPCR and sequencing data, binding sites were found in four other lncRNAs (MSTRG.34610, MSTRG.10941, MSTRG.81281 and MSTRG.93731) with AjamiR-2013-3p, a key miRNA that responds to hypoxia in sea cucumbers. The hypoxia-inducible factor (HIF-1 alpha) was also shown as the possible targeted mRNA of Aja-miR-2013-3p. As indicated by a dual-luciferase reporter assay system, "HIF-1 alpha gene/Aja-miR-2013-3p/MSTRG.34610" network and the "HIF-1 alpha gene/Aja-miR-20133p/MSTRG.10941" networkmay play important roles in sea cucumbers under environmental stresses. Moreover, environmental stress altered the expression of multiple lncRNAs and mRNAs, thus affecting various biological processes in A. japonicus, including immunity, energy metabolism and the cell cycle. At the molecular level, more comprehensive responseswere elicited by the combined thermal/hypoxic stress treatment than by individual stresses alone in sea cucumbers. This study lays the groundwork for future research on molecular mechanisms of echinoderm responses to thermal and hypoxic stress in the context of global climate changes. (C) 2019 Elsevier B.V. All rights reserved.
关键词Global warming RNA sequencing Non-coding RNA Echinoderm Heat Anoxia
DOI10.1016/j.scitotenv.2019.136045
收录类别SCI
语种英语
资助项目National Key R&D Program of China[2018YFD0900105] ; National Natural Science Foundation of China[41776161] ; National Natural Science Foundation of China[41776162] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-09] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Youth Innovation Promotion Association CAS[2019209]
WOS研究方向Environmental Sciences & Ecology
WOS类目Environmental Sciences
WOS记录号WOS:000512281700049
出版者ELSEVIER
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/165536
专题海洋生态与环境科学重点实验室
通讯作者Sun, Lina; Yang, Hongsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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Huo, Da,Sun, Lina,Storey, Kenneth B.,et al. The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,709:15.
APA Huo, Da.,Sun, Lina.,Storey, Kenneth B..,Zhang, Libin.,Liu, Shilin.,...&Yang, Hongsheng.(2020).The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.SCIENCE OF THE TOTAL ENVIRONMENT,709,15.
MLA Huo, Da,et al."The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses".SCIENCE OF THE TOTAL ENVIRONMENT 709(2020):15.
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