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The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses
Huo, Da1,2,3,4; Sun, Lina1,2,3,4; Storey, Kenneth B.5; Zhang, Libin1,2,3,4; Liu, Shilin1,2,3,4; Sun, Jingchun1,2,3,4; Yang, Hongsheng1,2,3,4
2020-03-20
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume709Pages:15
Corresponding AuthorSun, Lina(sunlina@qdio.ac.cn) ; Yang, Hongsheng(hshyang@ms.qdio.ac.cn)
AbstractThe aquatic environment can be greatly impacted by thermal and hypoxic stresses, particularly caused by intensified global warming. Hence, there is an urgency to understand the response mechanisms of marine organisms to adverse environment. Although long non-coding RNAs (lncRNAs) are involved in many biological processes, their roles in stress responses still remain unclear. Here, differentially expressed (DE) lncRNAs and mRNAs were identified as responses to environmental stresses in the economically important sea cucumber, Apostichopus japonicus, and their potential roles were explored. Based on a total of 159, 355 and 495 significantly upregulated genes and 230, 518 and 647 significantly downregulated genes identified in the thermal, hypoxic and combination thermal + hypoxic stress treatments, respectively, we constructed DE-lncRNA-mRNA coexpression networks. Among the networks, eight shared pairs were identified from the three treatments, and based on the connectivity degree, MSTRG.27265, MSTRG.19729 and MSTRG.95524 were shown to be crucial lncRNAs. Among all the significantly changed lncRNAs identified by RT-qPCR and sequencing data, binding sites were found in four other lncRNAs (MSTRG.34610, MSTRG.10941, MSTRG.81281 and MSTRG.93731) with AjamiR-2013-3p, a key miRNA that responds to hypoxia in sea cucumbers. The hypoxia-inducible factor (HIF-1 alpha) was also shown as the possible targeted mRNA of Aja-miR-2013-3p. As indicated by a dual-luciferase reporter assay system, "HIF-1 alpha gene/Aja-miR-2013-3p/MSTRG.34610" network and the "HIF-1 alpha gene/Aja-miR-20133p/MSTRG.10941" networkmay play important roles in sea cucumbers under environmental stresses. Moreover, environmental stress altered the expression of multiple lncRNAs and mRNAs, thus affecting various biological processes in A. japonicus, including immunity, energy metabolism and the cell cycle. At the molecular level, more comprehensive responseswere elicited by the combined thermal/hypoxic stress treatment than by individual stresses alone in sea cucumbers. This study lays the groundwork for future research on molecular mechanisms of echinoderm responses to thermal and hypoxic stress in the context of global climate changes. (C) 2019 Elsevier B.V. All rights reserved.
KeywordGlobal warming RNA sequencing Non-coding RNA Echinoderm Heat Anoxia
DOI10.1016/j.scitotenv.2019.136045
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900105] ; National Natural Science Foundation of China[41776161] ; National Natural Science Foundation of China[41776162] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-09] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Youth Innovation Promotion Association CAS[2019209]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000512281700049
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165536
Collection海洋生态与环境科学重点实验室
Corresponding AuthorSun, Lina; Yang, Hongsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huo, Da,Sun, Lina,Storey, Kenneth B.,et al. The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,709:15.
APA Huo, Da.,Sun, Lina.,Storey, Kenneth B..,Zhang, Libin.,Liu, Shilin.,...&Yang, Hongsheng.(2020).The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.SCIENCE OF THE TOTAL ENVIRONMENT,709,15.
MLA Huo, Da,et al."The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses".SCIENCE OF THE TOTAL ENVIRONMENT 709(2020):15.
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